Morphometric parameters and photosynthetic performance of in vitro propagated pineapple

Pineapple (Ananas comosus) is a horticultural species of the Bromeliaceae family of high socioeconomic interest, widely cultivated around the world. The multiplication of pineapple seedlings in the field can be time-consuming, requiring a significant labor investment. The objective of this study was...

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Detalles Bibliográficos
Autores principales: Marchi, Marcelo S., Ornellas, Thiago S., Fritsche, Yohan, Guerra, Miguel Pedro, Stefenon, Valdir Marcos
Formato: Articulo
Lenguaje:Inglés
Publicado: 2022
Materias:
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/147661
Aporte de:
id I19-R120-10915-147661
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Agrarias
Ananas comosus
Bioreactor
Micropropagation
Photosynthesis
In vitro culture
Biorreator
Micropropagação
Fotossíntese
Cultura in vitro
spellingShingle Ciencias Agrarias
Ananas comosus
Bioreactor
Micropropagation
Photosynthesis
In vitro culture
Biorreator
Micropropagação
Fotossíntese
Cultura in vitro
Marchi, Marcelo S.
Ornellas, Thiago S.
Fritsche, Yohan
Guerra, Miguel Pedro
Stefenon, Valdir Marcos
Morphometric parameters and photosynthetic performance of in vitro propagated pineapple
topic_facet Ciencias Agrarias
Ananas comosus
Bioreactor
Micropropagation
Photosynthesis
In vitro culture
Biorreator
Micropropagação
Fotossíntese
Cultura in vitro
description Pineapple (Ananas comosus) is a horticultural species of the Bromeliaceae family of high socioeconomic interest, widely cultivated around the world. The multiplication of pineapple seedlings in the field can be time-consuming, requiring a significant labor investment. The objective of this study was to evaluate the performance of continuous and temporary immersion systems in the micropropagation scale-up of the species. Shoots were obtained from explants subcultured in flasks with gelled culture medium and without gas exchange. The shoots were transferred to liquid MS medium supplemented with 2mM NAA, and 4mM BAP, and cultivated in four different devices: sealed flasks, flasks with semipermeable gas membranes, RITA®, and twin-flasks. After 45 days of cultivation, plant growth, fresh mass increment, the stomatal density of the abaxial surface of the leaves, the maximum quantum yield of the photosystem II, and contents of chlorophyll and carotenoids were analyzed. Significant differences were observed in plant growth, stomatal density, and contents of chlorophyll and carotenoids. The twin-flasks and RITA® devices revealed better results in morphological parameters, such as plant growth and stomatal density, while the treatments in sealed flasks and with membrane stood out in the chlorophyll content.
format Articulo
Articulo
author Marchi, Marcelo S.
Ornellas, Thiago S.
Fritsche, Yohan
Guerra, Miguel Pedro
Stefenon, Valdir Marcos
author_facet Marchi, Marcelo S.
Ornellas, Thiago S.
Fritsche, Yohan
Guerra, Miguel Pedro
Stefenon, Valdir Marcos
author_sort Marchi, Marcelo S.
title Morphometric parameters and photosynthetic performance of in vitro propagated pineapple
title_short Morphometric parameters and photosynthetic performance of in vitro propagated pineapple
title_full Morphometric parameters and photosynthetic performance of in vitro propagated pineapple
title_fullStr Morphometric parameters and photosynthetic performance of in vitro propagated pineapple
title_full_unstemmed Morphometric parameters and photosynthetic performance of in vitro propagated pineapple
title_sort morphometric parameters and photosynthetic performance of in vitro propagated pineapple
publishDate 2022
url http://sedici.unlp.edu.ar/handle/10915/147661
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