B-Chronic Lymphocytic Leukemia Autophagyc Cell Death by the Use of Manganese Doped Zinc Oxide Nanoparticles and Photo-Dynamic Therapy

B-Chronic Lymphocytic Leukemia (B-CLL) usually follows an adverse, relentless clinical course by slowly developing drug resistance to fludarabine and other chemotherapeutic agents, as well as by acquiring new different genetic abnormalities. As B-CLL cells spontaneously produce high amounts of React...

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Autores principales: Peña Luengas, Sandra, Marín, Gustavo Horacio, Rodriguez Nieto, Felipe Jorge, Dreon, Marcos Sebastián, Roque, Gustavo, Núñez, Luis, Sánchez, Francisco Homero, Tarditti, Adrian, Schinella, Guillermo Raúl, Pistaccio, Luis, Goya, Rodolfo Gustavo, Tau, José María, Ichim, Thomas, Riordan, Neil, Rivera Montalvo, Luis, Mansilla, Eduardo
Formato: Articulo
Lenguaje:Inglés
Publicado: 2015
Materias:
Medicina
Ciencias Exactas
B-Chronic Lymphocytic Leukemia
Zinc nanoparticles
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/147234
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-147234
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Medicina
Ciencias Exactas
B-Chronic Lymphocytic Leukemia
Zinc nanoparticles
spellingShingle Medicina
Ciencias Exactas
B-Chronic Lymphocytic Leukemia
Zinc nanoparticles
Peña Luengas, Sandra
Marín, Gustavo Horacio
Rodriguez Nieto, Felipe Jorge
Dreon, Marcos Sebastián
Roque, Gustavo
Núñez, Luis
Sánchez, Francisco Homero
Tarditti, Adrian
Schinella, Guillermo Raúl
Pistaccio, Luis
Goya, Rodolfo Gustavo
Tau, José María
Ichim, Thomas
Riordan, Neil
Rivera Montalvo, Luis
Mansilla, Eduardo
B-Chronic Lymphocytic Leukemia Autophagyc Cell Death by the Use of Manganese Doped Zinc Oxide Nanoparticles and Photo-Dynamic Therapy
topic_facet Medicina
Ciencias Exactas
B-Chronic Lymphocytic Leukemia
Zinc nanoparticles
description B-Chronic Lymphocytic Leukemia (B-CLL) usually follows an adverse, relentless clinical course by slowly developing drug resistance to fludarabine and other chemotherapeutic agents, as well as by acquiring new different genetic abnormalities. As B-CLL cells spontaneously produce high amounts of Reactive Oxygen Species (ROS) having an altered redox state in relation to that of normal B lymphocytes, we decided to probe different metal Zinc nanoparticles (ZnNPs) and quantify the levels of Singlet Oxigen (SO) to see if variations of its intracellular concentrations could execute and accelerate deadly programs in leukemic cells rather than in normal B lymphocytes, when applied with Photodynamic Therapy (PDT). In this way, we developed and tested a variety of metal ZnNPs of which one made of 0.5% Manganese Doped Zinc Oxide (ZnO:Mn) was finally selected for further testing as it had the best fludarabine resistant B-CLL cells in vitro killing activity, specially when combined with PDT. An interesting and rapidly dying process of B-CLL cells, known as autophagy, was always seen under Transmission Electronic Microscopy (TEM) when incubated with these 0.5% Mn doped ZnO NPs. This phenomenon correlated well with those intracellular increases of SO when PDT was administered, and measured by a novel method first described by us. As this therapy seems to be very specific to fludarabine resistant B-CLL cells, producing almost no damage to normal lymphocytes, it could surely contribute in the near future as a new innovative targeted strategy to be delivered in the clinical setting for the definitive benefit of these bad prognostic patients.
format Articulo
Articulo
author Peña Luengas, Sandra
Marín, Gustavo Horacio
Rodriguez Nieto, Felipe Jorge
Dreon, Marcos Sebastián
Roque, Gustavo
Núñez, Luis
Sánchez, Francisco Homero
Tarditti, Adrian
Schinella, Guillermo Raúl
Pistaccio, Luis
Goya, Rodolfo Gustavo
Tau, José María
Ichim, Thomas
Riordan, Neil
Rivera Montalvo, Luis
Mansilla, Eduardo
author_facet Peña Luengas, Sandra
Marín, Gustavo Horacio
Rodriguez Nieto, Felipe Jorge
Dreon, Marcos Sebastián
Roque, Gustavo
Núñez, Luis
Sánchez, Francisco Homero
Tarditti, Adrian
Schinella, Guillermo Raúl
Pistaccio, Luis
Goya, Rodolfo Gustavo
Tau, José María
Ichim, Thomas
Riordan, Neil
Rivera Montalvo, Luis
Mansilla, Eduardo
author_sort Peña Luengas, Sandra
title B-Chronic Lymphocytic Leukemia Autophagyc Cell Death by the Use of Manganese Doped Zinc Oxide Nanoparticles and Photo-Dynamic Therapy
title_short B-Chronic Lymphocytic Leukemia Autophagyc Cell Death by the Use of Manganese Doped Zinc Oxide Nanoparticles and Photo-Dynamic Therapy
title_full B-Chronic Lymphocytic Leukemia Autophagyc Cell Death by the Use of Manganese Doped Zinc Oxide Nanoparticles and Photo-Dynamic Therapy
title_fullStr B-Chronic Lymphocytic Leukemia Autophagyc Cell Death by the Use of Manganese Doped Zinc Oxide Nanoparticles and Photo-Dynamic Therapy
title_full_unstemmed B-Chronic Lymphocytic Leukemia Autophagyc Cell Death by the Use of Manganese Doped Zinc Oxide Nanoparticles and Photo-Dynamic Therapy
title_sort b-chronic lymphocytic leukemia autophagyc cell death by the use of manganese doped zinc oxide nanoparticles and photo-dynamic therapy
publishDate 2015
url http://sedici.unlp.edu.ar/handle/10915/147234
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