Fluoroquinolone-Resistant <i>Streptococcus agalactiae</i> Invasive Isolates Recovered in Argentina

Background: <i>Streptococcus agalactiae</i> or group B <i>Streptococcus</i> (GBS) is an important pathogen in neonates and nonpregnant individuals. Epidemiological studies of GBS resistance to fluoroquinolones (FQs) in Latin America are scarce. This study aimed to determine t...

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Detalles Bibliográficos
Autores principales: Arias, Barbara, Kovacec, Verónica, Vigliarolo, Laura, Suárez, Mariana, Tersigni, Carina, Müller, Loana, Lopardo, Horacio Ángel, Bonofiglio, Laura, Mollerach, Marta
Formato: Articulo Preprint
Lenguaje:Inglés
Publicado: 2019
Materias:
Ciencias Exactas
Biología
antibiotic resistance
epidemiology
fluoroquinolones
Streptococcus agalactiae
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/146544
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-146544
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Biología
antibiotic resistance
epidemiology
fluoroquinolones
Streptococcus agalactiae
spellingShingle Ciencias Exactas
Biología
antibiotic resistance
epidemiology
fluoroquinolones
Streptococcus agalactiae
Arias, Barbara
Kovacec, Verónica
Vigliarolo, Laura
Suárez, Mariana
Tersigni, Carina
Müller, Loana
Lopardo, Horacio Ángel
Bonofiglio, Laura
Mollerach, Marta
Fluoroquinolone-Resistant <i>Streptococcus agalactiae</i> Invasive Isolates Recovered in Argentina
topic_facet Ciencias Exactas
Biología
antibiotic resistance
epidemiology
fluoroquinolones
Streptococcus agalactiae
description Background: <i>Streptococcus agalactiae</i> or group B <i>Streptococcus</i> (GBS) is an important pathogen in neonates and nonpregnant individuals. Epidemiological studies of GBS resistance to fluoroquinolones (FQs) in Latin America are scarce. This study aimed to determine the local prevalence of FQ resistance in the frame of a national, prospective multicenter study of invasive GBS infections and to investigate mechanisms of resistance, serotype distribution, and clonal relationships among resistant isolates. Methods: From July 2014 to July 2015, 162 invasive GBS isolates were collected from 86 health care centers in 32 Argentinean cities. All isolates were screened for FQ nonsusceptibility using a five-disc scheme: levofloxacin (LVX), ciprofloxacin, norfloxacin (NOR), ofloxacin, and pefloxacin (PF). LVX minimal inhibitory concentration (MIC) was determined by the agar dilution method. Sequencing of internal regions of gyrA and parC genes was performed. Capsular typing and genetic characterization of nonsusceptible isolates were assessed by latex agglutination, pulsed-field gel electrophoresis (PFGE), and multilocus sequence typing. Results: Twenty-four of one hundred sixty-two GBS isolates exhibited no inhibition zones to all tested FQs with an MIC range of 16–32 mg/L for LVX, and one isolate with MIC = 1 mg/L showed no inhibition zones around NOR and PF discs. In all resistant isolates, point mutations were detected in both genes. Serotype Ib was prevalent (88%). One PFGE type accounted for 84% of the FQ-resistant isolates and belonged to serotype Ib, sequence type 10. Conclusions: The prevalence of FQ resistance was 14.8% likely to be associated with dissemination of an ST10/serotype Ib clone. The unexpected high rate of resistance emphasizes the relevance for continuous surveillance of GBS epidemiology and antibiotic susceptibility.
format Articulo
Preprint
author Arias, Barbara
Kovacec, Verónica
Vigliarolo, Laura
Suárez, Mariana
Tersigni, Carina
Müller, Loana
Lopardo, Horacio Ángel
Bonofiglio, Laura
Mollerach, Marta
author_facet Arias, Barbara
Kovacec, Verónica
Vigliarolo, Laura
Suárez, Mariana
Tersigni, Carina
Müller, Loana
Lopardo, Horacio Ángel
Bonofiglio, Laura
Mollerach, Marta
author_sort Arias, Barbara
title Fluoroquinolone-Resistant <i>Streptococcus agalactiae</i> Invasive Isolates Recovered in Argentina
title_short Fluoroquinolone-Resistant <i>Streptococcus agalactiae</i> Invasive Isolates Recovered in Argentina
title_full Fluoroquinolone-Resistant <i>Streptococcus agalactiae</i> Invasive Isolates Recovered in Argentina
title_fullStr Fluoroquinolone-Resistant <i>Streptococcus agalactiae</i> Invasive Isolates Recovered in Argentina
title_full_unstemmed Fluoroquinolone-Resistant <i>Streptococcus agalactiae</i> Invasive Isolates Recovered in Argentina
title_sort fluoroquinolone-resistant <i>streptococcus agalactiae</i> invasive isolates recovered in argentina
publishDate 2019
url http://sedici.unlp.edu.ar/handle/10915/146544
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