Characterisation of symbiotically efficient alfalfa-nodulating rhizobia isolated from acid soils of Argentina and Uruguay

The diversity, growth and symbiotic behaviour of symbiotically efficient alfalfa-nodulating rhizobia isolated from acid soils of Argentina and Uruguay were analysed. Partial sequencing of the 16S rDNA indicated that these isolates belong to Sinorhizobium meliloti species. IS-fingerprinting analysis...

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Autores principales: Segundo, E., Martínez-Abarca, F., van Dillewijn, P., Fernández-López, M., Lagares, Antonio, Martinez-Drets, G., Niehaus, K., Pühler, A., Toro, N.
Formato: Articulo
Lenguaje:Inglés
Publicado: 1999
Materias:
Biología
Competitiveness
Insertion sequence
Fingerprinting
pH
Symbiosis
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/146373
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-146373
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Competitiveness
Insertion sequence
Fingerprinting
pH
Symbiosis
spellingShingle Biología
Competitiveness
Insertion sequence
Fingerprinting
pH
Symbiosis
Segundo, E.
Martínez-Abarca, F.
van Dillewijn, P.
Fernández-López, M.
Lagares, Antonio
Martinez-Drets, G.
Niehaus, K.
Pühler, A.
Toro, N.
Characterisation of symbiotically efficient alfalfa-nodulating rhizobia isolated from acid soils of Argentina and Uruguay
topic_facet Biología
Competitiveness
Insertion sequence
Fingerprinting
pH
Symbiosis
description The diversity, growth and symbiotic behaviour of symbiotically efficient alfalfa-nodulating rhizobia isolated from acid soils of Argentina and Uruguay were analysed. Partial sequencing of the 16S rDNA indicated that these isolates belong to Sinorhizobium meliloti species. IS-fingerprinting analysis revealed a high diversity among the isolates but some of them appear related to inoculant strains currently used in the region. The S. meliloti isolates showed a decreased growth rate with increasing acidity. They were, however, able to nodulate alfalfa at pH 5.6, but showed a delayed nodulation and decreased nodule number typical of S. meliloti strains. The impaired nodulation of S. meliloti at pH 5.6 did not result in a reduction of alfalfa dry matter production or nitrogen content. However, significant differences were observed for the relative symbiotic effectiveness of the strains analysed. LPU63 (Argentina) was the most effective among the isolates and exhibited a high nodulation competitiveness at both neutral and acidic pH. These results suggest that the isolate LPU63 may be a potential efficient inoculant for alfalfa in acid soils.
format Articulo
Articulo
author Segundo, E.
Martínez-Abarca, F.
van Dillewijn, P.
Fernández-López, M.
Lagares, Antonio
Martinez-Drets, G.
Niehaus, K.
Pühler, A.
Toro, N.
author_facet Segundo, E.
Martínez-Abarca, F.
van Dillewijn, P.
Fernández-López, M.
Lagares, Antonio
Martinez-Drets, G.
Niehaus, K.
Pühler, A.
Toro, N.
author_sort Segundo, E.
title Characterisation of symbiotically efficient alfalfa-nodulating rhizobia isolated from acid soils of Argentina and Uruguay
title_short Characterisation of symbiotically efficient alfalfa-nodulating rhizobia isolated from acid soils of Argentina and Uruguay
title_full Characterisation of symbiotically efficient alfalfa-nodulating rhizobia isolated from acid soils of Argentina and Uruguay
title_fullStr Characterisation of symbiotically efficient alfalfa-nodulating rhizobia isolated from acid soils of Argentina and Uruguay
title_full_unstemmed Characterisation of symbiotically efficient alfalfa-nodulating rhizobia isolated from acid soils of Argentina and Uruguay
title_sort characterisation of symbiotically efficient alfalfa-nodulating rhizobia isolated from acid soils of argentina and uruguay
publishDate 1999
url http://sedici.unlp.edu.ar/handle/10915/146373
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