Biosynthesis of Polyunsaturated Fatty Acids from the Linoleic Acid Family in Cultured Cells

HTC cells (designated HTC for hepatoma tissue culture) were derived from the ascites form of a rat-carried Morris hepatoma 7288 C (Thompson et al, 1966). Previous studies have revealed that cells of this kind are able to desaturate and elongate fatty acids. In this respect it was demonstrated that c...

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Autores principales: Tacconi de Alaniz, María Josefa, Tacconi de Gómez Dumm, Irma Nelva, Brenner, Rodolfo Roberto
Formato: Libro Capitulo de libro
Lenguaje:Inglés
Publicado: Springer 1977
Materias:
Ciencias Médicas
Bioquímica
Arachidonic acid synthesis
Linoleic acid
Cell lipids
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/146218
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-146218
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Médicas
Bioquímica
Arachidonic acid synthesis
Linoleic acid
Cell lipids
spellingShingle Ciencias Médicas
Bioquímica
Arachidonic acid synthesis
Linoleic acid
Cell lipids
Tacconi de Alaniz, María Josefa
Tacconi de Gómez Dumm, Irma Nelva
Brenner, Rodolfo Roberto
Biosynthesis of Polyunsaturated Fatty Acids from the Linoleic Acid Family in Cultured Cells
topic_facet Ciencias Médicas
Bioquímica
Arachidonic acid synthesis
Linoleic acid
Cell lipids
description HTC cells (designated HTC for hepatoma tissue culture) were derived from the ascites form of a rat-carried Morris hepatoma 7288 C (Thompson et al, 1966). Previous studies have revealed that cells of this kind are able to desaturate and elongate fatty acids. In this respect it was demonstrated that culture HTC cells preserved the ability to desaturate stearic to oleic acid (Δ9 desaturase), α-linolenic acid to octdeca-6, 9,12,15-tetraenoic acid (Δ6 desaturase), and eicosa-8,11,14-trienoic acid to arachidonic acid (Δ5 desaturase) (Alaniz et al, 1975). They are also able to convert α-linolenic acid to higher homologs with 5 and 6 double bonds by desaturation and elongation reactions. These results also proved the existence of Δ4 desaturase activity (Alaniz et al, 1975). However, it was shown that the cell cultured in Swim’s medium supplemented with serum possessed a very low capacity to convert labeled linoleic acid of the medium to arachidonic acid. Nevertheless, these tumor cells readily converted eicosa-8,11,14-trienoic acid to arachidonic acid (Alaniz et al, 1975; Gaspar et al, 1975). Therefore, the difficulty of these cells to synthesize arachidonic acid from linoleic acid may reside in a step previous to the Δ5 desaturation of eicosa-8, 11, 14-trienoic acid. This step could be a Δ6 desaturation of linoleic acid. The discrepancy between the biosynthesis of linoleic and α-linolenic acid series is difficult to explain considering that the same enzyme desaturates linoleic and ±-linolenic acids in Δ6 position (Brenner and Peluffo, 1966) (Brenner, 1971; Brenner, 1974) (Ninno et al, 1974). For this reason it was important to investigate the routes of arachidonic acid synthesis from labeled linoleic acid and the possible incorporation of labeled acids in cell lipids.
format Libro
Capitulo de libro
author Tacconi de Alaniz, María Josefa
Tacconi de Gómez Dumm, Irma Nelva
Brenner, Rodolfo Roberto
author_facet Tacconi de Alaniz, María Josefa
Tacconi de Gómez Dumm, Irma Nelva
Brenner, Rodolfo Roberto
author_sort Tacconi de Alaniz, María Josefa
title Biosynthesis of Polyunsaturated Fatty Acids from the Linoleic Acid Family in Cultured Cells
title_short Biosynthesis of Polyunsaturated Fatty Acids from the Linoleic Acid Family in Cultured Cells
title_full Biosynthesis of Polyunsaturated Fatty Acids from the Linoleic Acid Family in Cultured Cells
title_fullStr Biosynthesis of Polyunsaturated Fatty Acids from the Linoleic Acid Family in Cultured Cells
title_full_unstemmed Biosynthesis of Polyunsaturated Fatty Acids from the Linoleic Acid Family in Cultured Cells
title_sort biosynthesis of polyunsaturated fatty acids from the linoleic acid family in cultured cells
publisher Springer
publishDate 1977
url http://sedici.unlp.edu.ar/handle/10915/146218
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AT brennerrodolforoberto biosynthesisofpolyunsaturatedfattyacidsfromthelinoleicacidfamilyinculturedcells
bdutipo_str Repositorios
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