In vitro conversion of saturated to monounsaturated fatty acid by Ehrlich ascites cells

In this paper, evidence is presented on the capacity of Ehrlich ascites cells to synthesize in vitro monounsaturated fatty acids from radioactive palmitate. Localization of the double bond was determined by ozonolysis and subsequent reduction of the ozonides to aldesters followed by gas liquid chrom...

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Detalles Bibliográficos
Autores principales:	Mercuri, Osvaldo Francisco, De Tomás, María Elena, Antueno, Roberto Justo de
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	1981
Materias:	Bioquímica Ehrlich ascites cells Fatty acid
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/146070
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-146070
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Bioquímica Ehrlich ascites cells Fatty acid
spellingShingle	Bioquímica Ehrlich ascites cells Fatty acid Mercuri, Osvaldo Francisco De Tomás, María Elena Antueno, Roberto Justo de In vitro conversion of saturated to monounsaturated fatty acid by Ehrlich ascites cells
topic_facet	Bioquímica Ehrlich ascites cells Fatty acid
description	In this paper, evidence is presented on the capacity of Ehrlich ascites cells to synthesize in vitro monounsaturated fatty acids from radioactive palmitate. Localization of the double bond was determined by ozonolysis and subsequent reduction of the ozonides to aldesters followed by gas liquid chromatography. These results proved that Ehrlich ascites cells have a Δ9 desaturase that catalyzes the biosynthesis of palmitoleic acid from palmitic acid and oleic and vaccenic acid via elongation-desaturation and desaturation-elongation, respectively, using palmitic acid as substrate. Furthermore, it is shown that, as in the hepatic cells, Δ9 desaturase enzyme activity of the tumoral cells is associated with the endoplasmic reticulum. The electron transport components involved in the desaturase system, i.e., NADH-cytochrome bs reductase and NADH-cytochrome bs reductase, were also measured. The activities of these enzymes do not appear to be rate-limiting in the desaturase activity of these tumoral cells.
format	Articulo Articulo
author	Mercuri, Osvaldo Francisco De Tomás, María Elena Antueno, Roberto Justo de
author_facet	Mercuri, Osvaldo Francisco De Tomás, María Elena Antueno, Roberto Justo de
author_sort	Mercuri, Osvaldo Francisco
title	In vitro conversion of saturated to monounsaturated fatty acid by Ehrlich ascites cells
title_short	In vitro conversion of saturated to monounsaturated fatty acid by Ehrlich ascites cells
title_full	In vitro conversion of saturated to monounsaturated fatty acid by Ehrlich ascites cells
title_fullStr	In vitro conversion of saturated to monounsaturated fatty acid by Ehrlich ascites cells
title_full_unstemmed	In vitro conversion of saturated to monounsaturated fatty acid by Ehrlich ascites cells
title_sort	in vitro conversion of saturated to monounsaturated fatty acid by ehrlich ascites cells
publishDate	1981
url	http://sedici.unlp.edu.ar/handle/10915/146070
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bdutipo_str	Repositorios
_version_	1764820460536594435

In vitro conversion of saturated to monounsaturated fatty acid by Ehrlich ascites cells

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