Efecto de OPN3 y MC4R en las corrientes mediadas por Kir.1

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Encephalopsin (OPN3) is part of the opsin family of light-sensitive G-protein coupled receptors. OPN3 was discovered in deep brain regions, specifically the arcuate nucleus and the paraventricular nucleus of the hypothalamus, however there have been limited research in the functionality of OPN3. Oan...

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Detalles Bibliográficos
Autor principal: Pinto, Victoria
Formato: Objeto de conferencia
Lenguaje:Español
Publicado: 2022
Materias:
Neurociencia
Patch Clamp
Corrientes
Receptores
Currents
Receptors
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/145690
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-145690
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Español
topic Neurociencia
Patch Clamp
Corrientes
Receptores
Patch Clamp
Currents
Receptors
spellingShingle Neurociencia
Patch Clamp
Corrientes
Receptores
Patch Clamp
Currents
Receptors
Pinto, Victoria
Efecto de OPN3 y MC4R en las corrientes mediadas por Kir.1
topic_facet Neurociencia
Patch Clamp
Corrientes
Receptores
Patch Clamp
Currents
Receptors
description Encephalopsin (OPN3) is part of the opsin family of light-sensitive G-protein coupled receptors. OPN3 was discovered in deep brain regions, specifically the arcuate nucleus and the paraventricular nucleus of the hypothalamus, however there have been limited research in the functionality of OPN3. Oancea lab has discovered that OPN3 functions in a light independent manner and signals through Gai to negatively regulate the melanogenic Gαs-coupled MC1R in human epidermal melanocytes. Neuronal melanocortin receptors (MC3R and MC4R) are found in the arcuate nucleus and paraventricular nucleus of the hypothalamus and play non-redundant roles in mediating energy balance. Both MC3R and MC4R share similar structural identities to MC1R and have been found to couple to Gαs. Additionally, Cone and his colleagues found that MC4R couples to Kir7.1 in hypothalamic neurons independent of G-protein activity. Previous data in our lab has revealed that OPN3 colocalizes and forms a physical complex with MC4R. The goal of this study is to test the hypothesis that OPN3 functions to negatively modulate MC4R-mediated signaling. More specifically, we overexpress OPN3 and MC4R in HEK293 cells together with Kir7.1 and measured the potassium current at diferent potentials. We only observed a significant amount of current in cells coexpressing OPN3 and this effect seems to be occluded by MC4R addition. More experiments are required to fully understand the signaling cascade involved and what is the effect of MC4R agonists.
format Objeto de conferencia
Objeto de conferencia
author Pinto, Victoria
author_facet Pinto, Victoria
author_sort Pinto, Victoria
title Efecto de OPN3 y MC4R en las corrientes mediadas por Kir.1
title_short Efecto de OPN3 y MC4R en las corrientes mediadas por Kir.1
title_full Efecto de OPN3 y MC4R en las corrientes mediadas por Kir.1
title_fullStr Efecto de OPN3 y MC4R en las corrientes mediadas por Kir.1
title_full_unstemmed Efecto de OPN3 y MC4R en las corrientes mediadas por Kir.1
title_sort efecto de opn3 y mc4r en las corrientes mediadas por kir.1
publishDate 2022
url http://sedici.unlp.edu.ar/handle/10915/145690
work_keys_str_mv AT pintovictoria efectodeopn3ymc4renlascorrientesmediadasporkir1
AT pintovictoria effectofopn3andmc4ronkir71mediatedcurrents
bdutipo_str Repositorios
_version_ 1764820461454098436

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