Physical and genetic map of Epinotia aporema granulovirus genome

The bean shoot borer, Epinotia aporema (Lep. Tortricidae), is an economically important pest of legume crops in South America. Recently, a granulovirus (EpapGV) was isolated from E. aporema larvae, and evaluated as a potential biological control agent. In order to generate a restriction map and to i...

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Detalles Bibliográficos
Autores principales: Parola, Alejandro Daniel, Manzán, María Alejandra, Lozano, Mario Enrique, Ghiringhelli, P. Daniel, Sciocco-Cap, Alicia, Romanowski, Víctor
Formato: Articulo
Lenguaje:Inglés
Publicado: 2002
Materias:
Biología
Baculovirus
EpapGV
Epinotia aporema
Granulin
Granulovirus
Phylogeny
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/145299
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-145299
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Baculovirus
EpapGV
Epinotia aporema
Granulin
Granulovirus
Phylogeny
spellingShingle Biología
Baculovirus
EpapGV
Epinotia aporema
Granulin
Granulovirus
Phylogeny
Parola, Alejandro Daniel
Manzán, María Alejandra
Lozano, Mario Enrique
Ghiringhelli, P. Daniel
Sciocco-Cap, Alicia
Romanowski, Víctor
Physical and genetic map of Epinotia aporema granulovirus genome
topic_facet Biología
Baculovirus
EpapGV
Epinotia aporema
Granulin
Granulovirus
Phylogeny
description The bean shoot borer, Epinotia aporema (Lep. Tortricidae), is an economically important pest of legume crops in South America. Recently, a granulovirus (EpapGV) was isolated from E. aporema larvae, and evaluated as a potential biological control agent. In order to generate a restriction map and to investigate the gene organisation of EpapGV genome, DNA isolated from occlusion bodies as well as a set of cloned genomic fragments were analysed using combinations of restriction endonucleases and Southern blot analyses that lead to a first version of the physical map. It was subsequently confirmed and refined by sequencing the termini of the cloned fragments and assessing their contiguity by comparing the sequences with databases to identify putative ORFs spanning neighbour fragments. This was also aided by PCR amplifications with primers that pointed outwards of the cloned viral DNA. The granulin gene was positioned on the physical map, cloned and sequenced. Its 747-nucleotide-long ORF encodes a predicted protein of 29 kDa and the core of the baculovirus very late promoter ATAAG was found 29 nucleotides upstream the initiation codon. In addition, 27 putative ORFs were located on the map and used to explore the genome organisation by GeneParityPlot against the fully sequenced granulovirus genomes. These data, taken together with the phylogenetic tree generated by alignment of the major occlusion proteins, indicate that EpapGV is closely related to CpGV, but has a distinct gene organisation.
format Articulo
Articulo
author Parola, Alejandro Daniel
Manzán, María Alejandra
Lozano, Mario Enrique
Ghiringhelli, P. Daniel
Sciocco-Cap, Alicia
Romanowski, Víctor
author_facet Parola, Alejandro Daniel
Manzán, María Alejandra
Lozano, Mario Enrique
Ghiringhelli, P. Daniel
Sciocco-Cap, Alicia
Romanowski, Víctor
author_sort Parola, Alejandro Daniel
title Physical and genetic map of Epinotia aporema granulovirus genome
title_short Physical and genetic map of Epinotia aporema granulovirus genome
title_full Physical and genetic map of Epinotia aporema granulovirus genome
title_fullStr Physical and genetic map of Epinotia aporema granulovirus genome
title_full_unstemmed Physical and genetic map of Epinotia aporema granulovirus genome
title_sort physical and genetic map of epinotia aporema granulovirus genome
publishDate 2002
url http://sedici.unlp.edu.ar/handle/10915/145299
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