Lectin-histochemistry: glycogenosis in cattle

Ten out of 47 calves that were born in a small Brahman herd from southern Brazil developed progressive muscular weakness and tremors, lethargy and poor body condition. Necropsy was performed on three affected animals. The only gross lesion detected was paleness of the muscles of the trunk and limbs....

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Autores principales:	Zlotowski, P., Gimeno, Eduardo Juan, Díaz, A., Barros, R., Barros, Severo S., Farias da Cruz, Claudio Estevao, Driemeier, D.
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2006
Materias:	Ciencias Veterinarias Brahman cattle generalized glycogenosis lectin histochemistry lysosomal storage disorder
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/145221
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-145221
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Ciencias Veterinarias Brahman cattle generalized glycogenosis lectin histochemistry lysosomal storage disorder
spellingShingle	Ciencias Veterinarias Brahman cattle generalized glycogenosis lectin histochemistry lysosomal storage disorder Zlotowski, P. Gimeno, Eduardo Juan Díaz, A. Barros, R. Barros, Severo S. Farias da Cruz, Claudio Estevao Driemeier, D. Lectin-histochemistry: glycogenosis in cattle
topic_facet	Ciencias Veterinarias Brahman cattle generalized glycogenosis lectin histochemistry lysosomal storage disorder
description	Ten out of 47 calves that were born in a small Brahman herd from southern Brazil developed progressive muscular weakness and tremors, lethargy and poor body condition. Necropsy was performed on three affected animals. The only gross lesion detected was paleness of the muscles of the trunk and limbs. Multiple cytoplasm vacuoles located in different tissues were the principal microscopic lesions. Vacuoles were particularly evident in skeletal muscles and myocardium. PAS-positive granules were numerous in skeletal muscle fibres and Purkinje fibres of the myocardium, but were also observed in the neurons of the brain and spinal cord, and in the vascular smooth muscle fibres from all the examined tissues. Pretreatment with diastase completely abolished the PAS reactivity. The vacuoles reacted strongly to Griffonia simplicifolia II and Concanavalia ensiformes lectins, whose biding pattern has been reported as useful for demonstration of glycogen. Examination of the electron micrographs revealed that glycogen was free within the cytoplasm or accumulated in membrane-bound granules of several tissues, especially in striated muscle, liver and neurons of the CNS. These findings were consistent with generalized glycogenosis.
format	Articulo Articulo
author	Zlotowski, P. Gimeno, Eduardo Juan Díaz, A. Barros, R. Barros, Severo S. Farias da Cruz, Claudio Estevao Driemeier, D.
author_facet	Zlotowski, P. Gimeno, Eduardo Juan Díaz, A. Barros, R. Barros, Severo S. Farias da Cruz, Claudio Estevao Driemeier, D.
author_sort	Zlotowski, P.
title	Lectin-histochemistry: glycogenosis in cattle
title_short	Lectin-histochemistry: glycogenosis in cattle
title_full	Lectin-histochemistry: glycogenosis in cattle
title_fullStr	Lectin-histochemistry: glycogenosis in cattle
title_full_unstemmed	Lectin-histochemistry: glycogenosis in cattle
title_sort	lectin-histochemistry: glycogenosis in cattle
publishDate	2006
url	http://sedici.unlp.edu.ar/handle/10915/145221
work_keys_str_mv	AT zlotowskip lectinhistochemistryglycogenosisincattle AT gimenoeduardojuan lectinhistochemistryglycogenosisincattle AT diaza lectinhistochemistryglycogenosisincattle AT barrosr lectinhistochemistryglycogenosisincattle AT barrosseveros lectinhistochemistryglycogenosisincattle AT fariasdacruzclaudioestevao lectinhistochemistryglycogenosisincattle AT driemeierd lectinhistochemistryglycogenosisincattle
bdutipo_str	Repositorios
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Lectin-histochemistry: glycogenosis in cattle

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