An indirect method for the rapid estimation of carotenoid contents in Phaeodactylum tricornutum : Possible application to other marine algae

The equations of Parsons and Strickland (1963) have been applied to calculate the concentration of total carotenoids in Phaeodactylum tricornutum, and the same pigments have simultaneously been determined on the individual fractions after TLC (thinlayer chromatography) separation. Comparison of both...

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Detalles Bibliográficos
Autores principales: Carreto, J. I., Catoggio, José Alberto
Formato: Articulo
Lenguaje:Inglés
Publicado: 1977
Materias:
Ciencias Exactas
Química
Chromatography
Chlorophyll
Carotenoid
Thinlayer Chromatography
Indirect Method
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/145154
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-145154
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Química
Chromatography
Chlorophyll
Carotenoid
Thinlayer Chromatography
Indirect Method
spellingShingle Ciencias Exactas
Química
Chromatography
Chlorophyll
Carotenoid
Thinlayer Chromatography
Indirect Method
Carreto, J. I.
Catoggio, José Alberto
An indirect method for the rapid estimation of carotenoid contents in Phaeodactylum tricornutum : Possible application to other marine algae
topic_facet Ciencias Exactas
Química
Chromatography
Chlorophyll
Carotenoid
Thinlayer Chromatography
Indirect Method
description The equations of Parsons and Strickland (1963) have been applied to calculate the concentration of total carotenoids in Phaeodactylum tricornutum, and the same pigments have simultaneously been determined on the individual fractions after TLC (thinlayer chromatography) separation. Comparison of both sets of data shows that for diverse carotenoids: chlorophyll a ratios, the direct spectrophotometric values range from 80 to 130% of the chromatographic values; wide variations in the proportions of different carotenoids account for such difference. On the basis of correlations between the proportion of each carotenoid and the absorbance ratio 480:663 nm, an indirect method for the rapid estimation of individual carotenoids of P. tricornutum is proposed. Its eventual application to other Chrysophyta species and to sea water is discussed.
format Articulo
Articulo
author Carreto, J. I.
Catoggio, José Alberto
author_facet Carreto, J. I.
Catoggio, José Alberto
author_sort Carreto, J. I.
title An indirect method for the rapid estimation of carotenoid contents in Phaeodactylum tricornutum : Possible application to other marine algae
title_short An indirect method for the rapid estimation of carotenoid contents in Phaeodactylum tricornutum : Possible application to other marine algae
title_full An indirect method for the rapid estimation of carotenoid contents in Phaeodactylum tricornutum : Possible application to other marine algae
title_fullStr An indirect method for the rapid estimation of carotenoid contents in Phaeodactylum tricornutum : Possible application to other marine algae
title_full_unstemmed An indirect method for the rapid estimation of carotenoid contents in Phaeodactylum tricornutum : Possible application to other marine algae
title_sort indirect method for the rapid estimation of carotenoid contents in phaeodactylum tricornutum : possible application to other marine algae
publishDate 1977
url http://sedici.unlp.edu.ar/handle/10915/145154
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