PCR detection of Dirofilaria immitis in <i>Aedes aegypti</i> and <i>Culex pipiens</i> from urban temperate Argentina

Dirofilariasis, a mosquito-borne disease of dogs caused by the nematode Dirofilaria immitis (Leidy; Spirurida: Onchocercidae), has now become a growing zoonotic concern. Based on direct microscopical observation, Aedes aegypti (L.) and Culex pipiens L. (Diptera: Culicidae) have been previously incri...

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Detalles Bibliográficos
Autores principales: Vezzani, Darío, Mesplet, María, Eiras, Diego Fernando, Fontanarrosa, María F., Schnittger, Leonhard
Formato: Articulo
Lenguaje:Inglés
Publicado: 2010
Materias:
Ciencias Veterinarias
Dirofilariasis
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/145115
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-145115
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Veterinarias
Dirofilariasis
spellingShingle Ciencias Veterinarias
Dirofilariasis
Vezzani, Darío
Mesplet, María
Eiras, Diego Fernando
Fontanarrosa, María F.
Schnittger, Leonhard
PCR detection of Dirofilaria immitis in <i>Aedes aegypti</i> and <i>Culex pipiens</i> from urban temperate Argentina
topic_facet Ciencias Veterinarias
Dirofilariasis
description Dirofilariasis, a mosquito-borne disease of dogs caused by the nematode Dirofilaria immitis (Leidy; Spirurida: Onchocercidae), has now become a growing zoonotic concern. Based on direct microscopical observation, Aedes aegypti (L.) and Culex pipiens L. (Diptera: Culicidae) have been previously incriminated as potential vectors of D. immitis in urban temperate Argentina. In this study, an effort was made to provide evidence for this assumption by screening of mosquitoes for D. immitis infection using a polymerase chain reaction (PCR) assay. PCR primers were developed to specifically amplify the D. immitis-16S rRNA gene and to reliably detect 100th of the genomic equivalent (10 pg) of the infective third-stage larvae in mosquito pools of up to 30 individuals. Collection of mosquitoes was performed between September 2007 and April 2008 in premises known to be inhabited by D. immitis-infected dogs in Greater Buenos Aires. The final collection comprised 453 specimens belonging to 11 mosquito species of the genera Aedes, Culex, Ochlerotatus, and Psorophora. PCR assays were performed on 82 pools (n ≤ 20) of heads and abdomens separately, as this allows differentiating infective and non-infective stages of the parasite, respectively. Identification of the non-infective stage of D. immitis in A. aegypti and C. pipiens provided additional strong support of transmission of the parasite by these species. To our knowledge, this was the first PCR screening for D. immitis-infected mosquitoes in South America.
format Articulo
Articulo
author Vezzani, Darío
Mesplet, María
Eiras, Diego Fernando
Fontanarrosa, María F.
Schnittger, Leonhard
author_facet Vezzani, Darío
Mesplet, María
Eiras, Diego Fernando
Fontanarrosa, María F.
Schnittger, Leonhard
author_sort Vezzani, Darío
title PCR detection of Dirofilaria immitis in <i>Aedes aegypti</i> and <i>Culex pipiens</i> from urban temperate Argentina
title_short PCR detection of Dirofilaria immitis in <i>Aedes aegypti</i> and <i>Culex pipiens</i> from urban temperate Argentina
title_full PCR detection of Dirofilaria immitis in <i>Aedes aegypti</i> and <i>Culex pipiens</i> from urban temperate Argentina
title_fullStr PCR detection of Dirofilaria immitis in <i>Aedes aegypti</i> and <i>Culex pipiens</i> from urban temperate Argentina
title_full_unstemmed PCR detection of Dirofilaria immitis in <i>Aedes aegypti</i> and <i>Culex pipiens</i> from urban temperate Argentina
title_sort pcr detection of dirofilaria immitis in <i>aedes aegypti</i> and <i>culex pipiens</i> from urban temperate argentina
publishDate 2010
url http://sedici.unlp.edu.ar/handle/10915/145115
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