Fenitrothion-Induced Structural and Functional Perturbations in the Yolk Lipoproteins of the Shrimp <i>Macrobrachium borellii</i>

Two lipovitellin (LV) forms containing the same apoproteins but differing in their lipid composition were isolated from <i>Macrobrachium borelii</i> eggs at early (LVe) and late (LVI) embryogenic stages and characterized. These two forms of LV, as well as liposomes prepared with lipids e...

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Detalles Bibliográficos
Autores principales: García, Carlos Fernando, González Baró, María del Rosario, Garda, Horacio Alberto, Cunningham, Mónica Liliana, Pollero, Ricardo José
Formato: Articulo
Lenguaje:Inglés
Publicado: 2004
Materias:
Ciencias Exactas
Bioquímica
Macrobrachium borellii
lipovitellin
fenitrothion
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/145027
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-145027
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Bioquímica
Macrobrachium borellii
lipovitellin
fenitrothion
spellingShingle Ciencias Exactas
Bioquímica
Macrobrachium borellii
lipovitellin
fenitrothion
García, Carlos Fernando
González Baró, María del Rosario
Garda, Horacio Alberto
Cunningham, Mónica Liliana
Pollero, Ricardo José
Fenitrothion-Induced Structural and Functional Perturbations in the Yolk Lipoproteins of the Shrimp <i>Macrobrachium borellii</i>
topic_facet Ciencias Exactas
Bioquímica
Macrobrachium borellii
lipovitellin
fenitrothion
description Two lipovitellin (LV) forms containing the same apoproteins but differing in their lipid composition were isolated from <i>Macrobrachium borelii</i> eggs at early (LVe) and late (LVI) embryogenic stages and characterized. These two forms of LV, as well as liposomes prepared with lipids extracted from them, were used as simpler models to study the effect of the pesticide fenitrothion (FS) on their structures and functions. Rotational diffusion and fluorescence lifetime of two fluorescent probes [1,6-diphenyl-1,3,5-hexatriene (DPH) and 3-(p-(6-phenyl)-1,3,5-hexatrienal)phenylpropionic acid (DPH-PA)] were used to obtain information on structural changes induced by FS in the inner and outer regions of the LV, respectively. Comparison of the rotational behavior of these probes in native LV and liposomes (LP) from extracted LV lipids suggests that apoprotein-lipid interactions result in an ordered neutral lipid core. FS increased the lipid phase polarity of both LV and LP forms. The rotation of these probes in LP was not affected, suggesting a dependence of FS action on lipid-protein interactions. DPH-PA steady-state anisotropy showed that, unlike the LVe form, the LVI form was sensitive to extremely low FS concentrations. The ability of both LV to transfer palmitic acid to albumin was increased, but in a dissimilar manner, by the presence of FS. Such differences in the sensitivity of the LV at different steps of embryogenesis to FS influence the toxic action of this insecticide.
format Articulo
Articulo
author García, Carlos Fernando
González Baró, María del Rosario
Garda, Horacio Alberto
Cunningham, Mónica Liliana
Pollero, Ricardo José
author_facet García, Carlos Fernando
González Baró, María del Rosario
Garda, Horacio Alberto
Cunningham, Mónica Liliana
Pollero, Ricardo José
author_sort García, Carlos Fernando
title Fenitrothion-Induced Structural and Functional Perturbations in the Yolk Lipoproteins of the Shrimp <i>Macrobrachium borellii</i>
title_short Fenitrothion-Induced Structural and Functional Perturbations in the Yolk Lipoproteins of the Shrimp <i>Macrobrachium borellii</i>
title_full Fenitrothion-Induced Structural and Functional Perturbations in the Yolk Lipoproteins of the Shrimp <i>Macrobrachium borellii</i>
title_fullStr Fenitrothion-Induced Structural and Functional Perturbations in the Yolk Lipoproteins of the Shrimp <i>Macrobrachium borellii</i>
title_full_unstemmed Fenitrothion-Induced Structural and Functional Perturbations in the Yolk Lipoproteins of the Shrimp <i>Macrobrachium borellii</i>
title_sort fenitrothion-induced structural and functional perturbations in the yolk lipoproteins of the shrimp <i>macrobrachium borellii</i>
publishDate 2004
url http://sedici.unlp.edu.ar/handle/10915/145027
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