One-step concentration and partial purification of <i>Aspergillus kawachii</i> non-acidic polygalacturonases by adsorption to glass fiber microfilters

The non-acidic polygalacturonases produced by <i>Aspergillus kawachii</i> in a glucose/tryptone medium were adsorbed to a glass fiber microfilter that was used to clarify the fermentation broth. Maximum adsorption occurred at pH 3 under low ionic strength conditions. The adsorbed activit...

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Autores principales: Voget, Claudio Enrique, Vita, Carolina Elena, Contreras Esquivel, Juan Carlos
Formato: Articulo
Lenguaje:Inglés
Publicado: 2006
Materias:
Ciencias Exactas
Química
Aspergillus kawachii
glass fiber microfilter
polygalacturonases
protein adsorption
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/145008
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-145008
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Química
Aspergillus kawachii
glass fiber microfilter
polygalacturonases
protein adsorption
spellingShingle Ciencias Exactas
Química
Aspergillus kawachii
glass fiber microfilter
polygalacturonases
protein adsorption
Voget, Claudio Enrique
Vita, Carolina Elena
Contreras Esquivel, Juan Carlos
One-step concentration and partial purification of <i>Aspergillus kawachii</i> non-acidic polygalacturonases by adsorption to glass fiber microfilters
topic_facet Ciencias Exactas
Química
Aspergillus kawachii
glass fiber microfilter
polygalacturonases
protein adsorption
description The non-acidic polygalacturonases produced by <i>Aspergillus kawachii</i> in a glucose/tryptone medium were adsorbed to a glass fiber microfilter that was used to clarify the fermentation broth. Maximum adsorption occurred at pH 3 under low ionic strength conditions. The adsorbed activity could be readily released with a buffer solution at pH 5. Based upon these observations, a separation process was developed which enabled the broth to be clarified and, at the same time, the non-acidic polygalacturonases to be concentrated 20-fold and purified 100-fold in a unique filtration step. The practical advantage of recovering polygalacturonases by a filtration process lies in the simplicity and efficiency of the operation involved.
format Articulo
Articulo
author Voget, Claudio Enrique
Vita, Carolina Elena
Contreras Esquivel, Juan Carlos
author_facet Voget, Claudio Enrique
Vita, Carolina Elena
Contreras Esquivel, Juan Carlos
author_sort Voget, Claudio Enrique
title One-step concentration and partial purification of <i>Aspergillus kawachii</i> non-acidic polygalacturonases by adsorption to glass fiber microfilters
title_short One-step concentration and partial purification of <i>Aspergillus kawachii</i> non-acidic polygalacturonases by adsorption to glass fiber microfilters
title_full One-step concentration and partial purification of <i>Aspergillus kawachii</i> non-acidic polygalacturonases by adsorption to glass fiber microfilters
title_fullStr One-step concentration and partial purification of <i>Aspergillus kawachii</i> non-acidic polygalacturonases by adsorption to glass fiber microfilters
title_full_unstemmed One-step concentration and partial purification of <i>Aspergillus kawachii</i> non-acidic polygalacturonases by adsorption to glass fiber microfilters
title_sort one-step concentration and partial purification of <i>aspergillus kawachii</i> non-acidic polygalacturonases by adsorption to glass fiber microfilters
publishDate 2006
url http://sedici.unlp.edu.ar/handle/10915/145008
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