IL-10 expression by CT26 colon carcinoma cells inhibits their malignant phenotype and induces a T cell-mediated tumor rejection in the context of a systemic Th2 response

In spite of the evidence that IL-10 has Th1-immunosuppressive and anti-inflammatory effects, it has been shown that IL-10 may reduce the tumorigenic capacity of certain tumor cell types. In order to characterize the responses elicited by IL-10, we explored the effect of transducing murine CT26 colon...

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Autores principales: Adris, Soraya, Klein, S., Jasnis, M A, Chuluyan, E, Ledda, M. F., Bravo, Alicia I., Carbone, Cecilia, Chernajovsky, Yuti, Podhajcer, Osvaldo L.
Formato: Articulo
Lenguaje:Inglés
Publicado: 1999
Materias:
Bioquímica
Gene transfer
Cytokines
Interleukin-10
Tumor immunity
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/144188
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-144188
record_format dspace
spelling I19-R120-10915-1441882024-10-21T18:28:16Z http://sedici.unlp.edu.ar/handle/10915/144188 IL-10 expression by CT26 colon carcinoma cells inhibits their malignant phenotype and induces a T cell-mediated tumor rejection in the context of a systemic Th2 response Adris, Soraya Klein, S. Jasnis, M A Chuluyan, E Ledda, M. F. Bravo, Alicia I. Carbone, Cecilia Chernajovsky, Yuti Podhajcer, Osvaldo L. 1999 2022-10-20T16:42:48Z en Bioquímica Gene transfer Cytokines Interleukin-10 Tumor immunity In spite of the evidence that IL-10 has Th1-immunosuppressive and anti-inflammatory effects, it has been shown that IL-10 may reduce the tumorigenic capacity of certain tumor cell types. In order to characterize the responses elicited by IL-10, we explored the effect of transducing murine CT26 colon carcinoma cells with a recombinant retrovirus expressing mIL-10. IL-10 gene transfer of CT26 cells had no effect on tumor cell growth on plastic surface but inhibited the anchorage-independent growth capacity of tumor cells and their metastatic potential as assessed by their invasive and migration ability. Expression of IL-10 also elicited an antitumor immune response involving both CD4+ and CD8+ T cells. Assessment of the immune status of the animals demonstrated that mice injected with CT26-IL10 cells showed prevalence of a systemic and tumor-specific Th2 response. Spleen cells obtained from these mice showed an increased production of IL-4 and no changes in IFNgamma levels, characteristic of a Th2 response. These results demonstrate that IL-10 affects CT26 tumor cell growth by both inhibiting the malignant phenotype and by recruiting and activating a T cell-mediated antitumor response. This T cell response occurs in the context of a shift towards a Th2 response. Facultad de Ciencias Veterinarias Articulo Articulo http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf 1705-1712
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Bioquímica
Gene transfer
Cytokines
Interleukin-10
Tumor immunity
spellingShingle Bioquímica
Gene transfer
Cytokines
Interleukin-10
Tumor immunity
Adris, Soraya
Klein, S.
Jasnis, M A
Chuluyan, E
Ledda, M. F.
Bravo, Alicia I.
Carbone, Cecilia
Chernajovsky, Yuti
Podhajcer, Osvaldo L.
IL-10 expression by CT26 colon carcinoma cells inhibits their malignant phenotype and induces a T cell-mediated tumor rejection in the context of a systemic Th2 response
topic_facet Bioquímica
Gene transfer
Cytokines
Interleukin-10
Tumor immunity
description In spite of the evidence that IL-10 has Th1-immunosuppressive and anti-inflammatory effects, it has been shown that IL-10 may reduce the tumorigenic capacity of certain tumor cell types. In order to characterize the responses elicited by IL-10, we explored the effect of transducing murine CT26 colon carcinoma cells with a recombinant retrovirus expressing mIL-10. IL-10 gene transfer of CT26 cells had no effect on tumor cell growth on plastic surface but inhibited the anchorage-independent growth capacity of tumor cells and their metastatic potential as assessed by their invasive and migration ability. Expression of IL-10 also elicited an antitumor immune response involving both CD4+ and CD8+ T cells. Assessment of the immune status of the animals demonstrated that mice injected with CT26-IL10 cells showed prevalence of a systemic and tumor-specific Th2 response. Spleen cells obtained from these mice showed an increased production of IL-4 and no changes in IFNgamma levels, characteristic of a Th2 response. These results demonstrate that IL-10 affects CT26 tumor cell growth by both inhibiting the malignant phenotype and by recruiting and activating a T cell-mediated antitumor response. This T cell response occurs in the context of a shift towards a Th2 response.
format Articulo
Articulo
author Adris, Soraya
Klein, S.
Jasnis, M A
Chuluyan, E
Ledda, M. F.
Bravo, Alicia I.
Carbone, Cecilia
Chernajovsky, Yuti
Podhajcer, Osvaldo L.
author_facet Adris, Soraya
Klein, S.
Jasnis, M A
Chuluyan, E
Ledda, M. F.
Bravo, Alicia I.
Carbone, Cecilia
Chernajovsky, Yuti
Podhajcer, Osvaldo L.
author_sort Adris, Soraya
title IL-10 expression by CT26 colon carcinoma cells inhibits their malignant phenotype and induces a T cell-mediated tumor rejection in the context of a systemic Th2 response
title_short IL-10 expression by CT26 colon carcinoma cells inhibits their malignant phenotype and induces a T cell-mediated tumor rejection in the context of a systemic Th2 response
title_full IL-10 expression by CT26 colon carcinoma cells inhibits their malignant phenotype and induces a T cell-mediated tumor rejection in the context of a systemic Th2 response
title_fullStr IL-10 expression by CT26 colon carcinoma cells inhibits their malignant phenotype and induces a T cell-mediated tumor rejection in the context of a systemic Th2 response
title_full_unstemmed IL-10 expression by CT26 colon carcinoma cells inhibits their malignant phenotype and induces a T cell-mediated tumor rejection in the context of a systemic Th2 response
title_sort il-10 expression by ct26 colon carcinoma cells inhibits their malignant phenotype and induces a t cell-mediated tumor rejection in the context of a systemic th2 response
publishDate 1999
url http://sedici.unlp.edu.ar/handle/10915/144188
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