Cross-linked α-l-rhamnosidase aggregates with potential application in food industry

α-l-Rhamnosidase (E.C. 3.2.1.40) from Brevundimonas sp. Ci19 was immobilized as a cross-linked enzyme aggregate (CLEA) via precipitation with acetone followed by glutaraldehyde cross-linking. The effects of precipitation and cross-linking on CLEA activity were investigated and characterized. Sixty p...

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Autores principales: Alvarenga, Adriana Elizabet, Amoroso, María Julia, Illanes, Andrés, Castro, Guillermo Raúl
Formato: Articulo
Lenguaje:Inglés
Publicado: 2014
Materias:
Biología
Immobilization
α-l-Rhamnosidase
Brevundimonas sp. Ci19
Cross-linked enzyme aggregate (CLEA)
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/143998
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-143998
record_format dspace
spelling I19-R120-10915-1439982023-05-10T18:58:38Z http://sedici.unlp.edu.ar/handle/10915/143998 issn:1438-2377 issn:1438-2385 Cross-linked α-l-rhamnosidase aggregates with potential application in food industry Alvarenga, Adriana Elizabet Amoroso, María Julia Illanes, Andrés Castro, Guillermo Raúl 2014-01-22 2022-10-18T15:07:00Z en Biología Immobilization α-l-Rhamnosidase Brevundimonas sp. Ci19 Cross-linked enzyme aggregate (CLEA) α-l-Rhamnosidase (E.C. 3.2.1.40) from Brevundimonas sp. Ci19 was immobilized as a cross-linked enzyme aggregate (CLEA) via precipitation with acetone followed by glutaraldehyde cross-linking. The effects of precipitation and cross-linking on CLEA activity were investigated and characterized. Sixty percent acetone solution and 2.0 % glutaraldehyde were used at pH 7.0 for 1-h cross-linking reaction. The yield of rhamnosidase-CLEA was approximately 80 % starting from crude extracts or pure enzyme suggesting non-purification steps are required for extended use. No significant differences in optimum pH and temperature values of the enzyme were recorded after immobilization. The rham-CLEA recycled 4 times showed about 80 % rhamnosidase activity; meanwhile, in the fifth and six recycled time, the enzyme activity was reduced to about 40 and 20 %, respectively. Centro de Investigación y Desarrollo en Fermentaciones Industriales Articulo Articulo http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf 797-801
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Immobilization
α-l-Rhamnosidase
Brevundimonas sp. Ci19
Cross-linked enzyme aggregate (CLEA)
spellingShingle Biología
Immobilization
α-l-Rhamnosidase
Brevundimonas sp. Ci19
Cross-linked enzyme aggregate (CLEA)
Alvarenga, Adriana Elizabet
Amoroso, María Julia
Illanes, Andrés
Castro, Guillermo Raúl
Cross-linked α-l-rhamnosidase aggregates with potential application in food industry
topic_facet Biología
Immobilization
α-l-Rhamnosidase
Brevundimonas sp. Ci19
Cross-linked enzyme aggregate (CLEA)
description α-l-Rhamnosidase (E.C. 3.2.1.40) from Brevundimonas sp. Ci19 was immobilized as a cross-linked enzyme aggregate (CLEA) via precipitation with acetone followed by glutaraldehyde cross-linking. The effects of precipitation and cross-linking on CLEA activity were investigated and characterized. Sixty percent acetone solution and 2.0 % glutaraldehyde were used at pH 7.0 for 1-h cross-linking reaction. The yield of rhamnosidase-CLEA was approximately 80 % starting from crude extracts or pure enzyme suggesting non-purification steps are required for extended use. No significant differences in optimum pH and temperature values of the enzyme were recorded after immobilization. The rham-CLEA recycled 4 times showed about 80 % rhamnosidase activity; meanwhile, in the fifth and six recycled time, the enzyme activity was reduced to about 40 and 20 %, respectively.
format Articulo
Articulo
author Alvarenga, Adriana Elizabet
Amoroso, María Julia
Illanes, Andrés
Castro, Guillermo Raúl
author_facet Alvarenga, Adriana Elizabet
Amoroso, María Julia
Illanes, Andrés
Castro, Guillermo Raúl
author_sort Alvarenga, Adriana Elizabet
title Cross-linked α-l-rhamnosidase aggregates with potential application in food industry
title_short Cross-linked α-l-rhamnosidase aggregates with potential application in food industry
title_full Cross-linked α-l-rhamnosidase aggregates with potential application in food industry
title_fullStr Cross-linked α-l-rhamnosidase aggregates with potential application in food industry
title_full_unstemmed Cross-linked α-l-rhamnosidase aggregates with potential application in food industry
title_sort cross-linked α-l-rhamnosidase aggregates with potential application in food industry
publishDate 2014
url http://sedici.unlp.edu.ar/handle/10915/143998
work_keys_str_mv AT alvarengaadrianaelizabet crosslinkedalrhamnosidaseaggregateswithpotentialapplicationinfoodindustry
AT amorosomariajulia crosslinkedalrhamnosidaseaggregateswithpotentialapplicationinfoodindustry
AT illanesandres crosslinkedalrhamnosidaseaggregateswithpotentialapplicationinfoodindustry
AT castroguillermoraul crosslinkedalrhamnosidaseaggregateswithpotentialapplicationinfoodindustry
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