Feasibility of using RFLP of PCR-amplified 16S rRNA gene(s) for rapid differentiation of isolates of aerobic spore-forming bacteria from honey

This study aimed to assess the feasibility of using RFLP of PCR-amplified 16S rRNA gene (s) by using universal primers 27f/1492r and a combination of three restriction enzymes, AluI, CfoI, and TaqI, for a low-cost, rapid screen for a primarily differentiation of isolates of the complex of aerobic sp...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: López, Ana Claudia, Alippi, Adriana Mónica
Formato: Articulo Preprint
Lenguaje:Inglés
Publicado: 2019
Materias:
Biología
PCR-RFLP
Bacillus
Paenibacillus
Brevibacillus
Lysinibacillus
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/143201
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-143201
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
PCR-RFLP
Bacillus
Paenibacillus
Brevibacillus
Lysinibacillus
spellingShingle Biología
PCR-RFLP
Bacillus
Paenibacillus
Brevibacillus
Lysinibacillus
López, Ana Claudia
Alippi, Adriana Mónica
Feasibility of using RFLP of PCR-amplified 16S rRNA gene(s) for rapid differentiation of isolates of aerobic spore-forming bacteria from honey
topic_facet Biología
PCR-RFLP
Bacillus
Paenibacillus
Brevibacillus
Lysinibacillus
description This study aimed to assess the feasibility of using RFLP of PCR-amplified 16S rRNA gene (s) by using universal primers 27f/1492r and a combination of three restriction enzymes, AluI, CfoI, and TaqI, for a low-cost, rapid screen for a primarily differentiation of isolates of the complex of aerobic spore-forming bacteria commonly found in honey samples. The described method produced unique and distinguishable patterns to differentiate among 80 isolates belonging to 26 different species of Bacillus, Brevibacillus, Lysinibacillus, Rummeliibacillus, and Paenibacillus reported in honey and other apiarian sources.
format Articulo
Preprint
author López, Ana Claudia
Alippi, Adriana Mónica
author_facet López, Ana Claudia
Alippi, Adriana Mónica
author_sort López, Ana Claudia
title Feasibility of using RFLP of PCR-amplified 16S rRNA gene(s) for rapid differentiation of isolates of aerobic spore-forming bacteria from honey
title_short Feasibility of using RFLP of PCR-amplified 16S rRNA gene(s) for rapid differentiation of isolates of aerobic spore-forming bacteria from honey
title_full Feasibility of using RFLP of PCR-amplified 16S rRNA gene(s) for rapid differentiation of isolates of aerobic spore-forming bacteria from honey
title_fullStr Feasibility of using RFLP of PCR-amplified 16S rRNA gene(s) for rapid differentiation of isolates of aerobic spore-forming bacteria from honey
title_full_unstemmed Feasibility of using RFLP of PCR-amplified 16S rRNA gene(s) for rapid differentiation of isolates of aerobic spore-forming bacteria from honey
title_sort feasibility of using rflp of pcr-amplified 16s rrna gene(s) for rapid differentiation of isolates of aerobic spore-forming bacteria from honey
publishDate 2019
url http://sedici.unlp.edu.ar/handle/10915/143201
work_keys_str_mv AT lopezanaclaudia feasibilityofusingrflpofpcramplified16srrnagenesforrapiddifferentiationofisolatesofaerobicsporeformingbacteriafromhoney
AT alippiadrianamonica feasibilityofusingrflpofpcramplified16srrnagenesforrapiddifferentiationofisolatesofaerobicsporeformingbacteriafromhoney
bdutipo_str Repositorios
_version_ 1764820459709267969

Ejemplares similares