Cytotoxic and genotoxic effects induced by enrofloxacin-based antibiotic formulation Floxagen® in two experimental models of bovine cells in vitro : Peripheral lymphocytes and cumulus cells

The in vitro effect of enrofloxacin (EFZ) was tested on two experimental somatic bovine cells in vitro: peripheral lymphocytes (PLs) and cumulus cells (CCs). The cytotoxicity and genotoxicity of this veterinary antibiotic were assessed using 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium b...

Descripción completa

Detalles Bibliográficos
Autores principales: Anchordoquy, Juan Patricio, Anchordoquy, Juan Mateo, Nikoloff, Noelia, Gambaro, Rocío Celeste, Padula, Gisel, Furnus, Cecilia Cristina, Seoane, Analía Isabel
Formato: Articulo
Lenguaje:Inglés
Publicado: 2018
Materias:
Veterinaria
Bovine cells
MTT
SCGE assay
CBMN cyt assay
Enrofloxacin
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/142354
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-142354
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Veterinaria
Bovine cells
MTT
SCGE assay
CBMN cyt assay
Enrofloxacin
spellingShingle Veterinaria
Bovine cells
MTT
SCGE assay
CBMN cyt assay
Enrofloxacin
Anchordoquy, Juan Patricio
Anchordoquy, Juan Mateo
Nikoloff, Noelia
Gambaro, Rocío Celeste
Padula, Gisel
Furnus, Cecilia Cristina
Seoane, Analía Isabel
Cytotoxic and genotoxic effects induced by enrofloxacin-based antibiotic formulation Floxagen® in two experimental models of bovine cells in vitro : Peripheral lymphocytes and cumulus cells
topic_facet Veterinaria
Bovine cells
MTT
SCGE assay
CBMN cyt assay
Enrofloxacin
description The in vitro effect of enrofloxacin (EFZ) was tested on two experimental somatic bovine cells in vitro: peripheral lymphocytes (PLs) and cumulus cells (CCs). The cytotoxicity and genotoxicity of this veterinary antibiotic were assessed using 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assays, single-cell gel electrophoresis (SCGE) assay, and cytokinesis-block micronucleus cytome (CBMN cyt) assay. Cells were treated during 24 h, and three concentrations were tested (50 μg/mL, 100 μg/mL, 150 μg/mL). When EFZ was tested in PLs, the results demonstrated that the antibiotic was able to induce cell death and DNA damage with all concentrations. In addition, 50 μg/mL and 100 μg/mL EFZ increased frequencies of micronuclei (MNi). On the other hand, the highest EFZ concentration occasioned cellular cytotoxicity in CCs as evidenced by mitochondrial activity alterations. Nevertheless, EFZ was not able to induce DNA damage and MNi in CCs. These results represent the first experimental evidence of genotoxic and cytotoxic effects exerted by EFZ in bovine PLs and CCs.
format Articulo
Articulo
author Anchordoquy, Juan Patricio
Anchordoquy, Juan Mateo
Nikoloff, Noelia
Gambaro, Rocío Celeste
Padula, Gisel
Furnus, Cecilia Cristina
Seoane, Analía Isabel
author_facet Anchordoquy, Juan Patricio
Anchordoquy, Juan Mateo
Nikoloff, Noelia
Gambaro, Rocío Celeste
Padula, Gisel
Furnus, Cecilia Cristina
Seoane, Analía Isabel
author_sort Anchordoquy, Juan Patricio
title Cytotoxic and genotoxic effects induced by enrofloxacin-based antibiotic formulation Floxagen® in two experimental models of bovine cells in vitro : Peripheral lymphocytes and cumulus cells
title_short Cytotoxic and genotoxic effects induced by enrofloxacin-based antibiotic formulation Floxagen® in two experimental models of bovine cells in vitro : Peripheral lymphocytes and cumulus cells
title_full Cytotoxic and genotoxic effects induced by enrofloxacin-based antibiotic formulation Floxagen® in two experimental models of bovine cells in vitro : Peripheral lymphocytes and cumulus cells
title_fullStr Cytotoxic and genotoxic effects induced by enrofloxacin-based antibiotic formulation Floxagen® in two experimental models of bovine cells in vitro : Peripheral lymphocytes and cumulus cells
title_full_unstemmed Cytotoxic and genotoxic effects induced by enrofloxacin-based antibiotic formulation Floxagen® in two experimental models of bovine cells in vitro : Peripheral lymphocytes and cumulus cells
title_sort cytotoxic and genotoxic effects induced by enrofloxacin-based antibiotic formulation floxagen® in two experimental models of bovine cells in vitro : peripheral lymphocytes and cumulus cells
publishDate 2018
url http://sedici.unlp.edu.ar/handle/10915/142354
work_keys_str_mv AT anchordoquyjuanpatricio cytotoxicandgenotoxiceffectsinducedbyenrofloxacinbasedantibioticformulationfloxagenintwoexperimentalmodelsofbovinecellsinvitroperipherallymphocytesandcumuluscells
AT anchordoquyjuanmateo cytotoxicandgenotoxiceffectsinducedbyenrofloxacinbasedantibioticformulationfloxagenintwoexperimentalmodelsofbovinecellsinvitroperipherallymphocytesandcumuluscells
AT nikoloffnoelia cytotoxicandgenotoxiceffectsinducedbyenrofloxacinbasedantibioticformulationfloxagenintwoexperimentalmodelsofbovinecellsinvitroperipherallymphocytesandcumuluscells
AT gambarorocioceleste cytotoxicandgenotoxiceffectsinducedbyenrofloxacinbasedantibioticformulationfloxagenintwoexperimentalmodelsofbovinecellsinvitroperipherallymphocytesandcumuluscells
AT padulagisel cytotoxicandgenotoxiceffectsinducedbyenrofloxacinbasedantibioticformulationfloxagenintwoexperimentalmodelsofbovinecellsinvitroperipherallymphocytesandcumuluscells
AT furnusceciliacristina cytotoxicandgenotoxiceffectsinducedbyenrofloxacinbasedantibioticformulationfloxagenintwoexperimentalmodelsofbovinecellsinvitroperipherallymphocytesandcumuluscells
AT seoaneanaliaisabel cytotoxicandgenotoxiceffectsinducedbyenrofloxacinbasedantibioticformulationfloxagenintwoexperimentalmodelsofbovinecellsinvitroperipherallymphocytesandcumuluscells
bdutipo_str Repositorios
_version_ 1764820459417763840

Ejemplares similares