Umbilical Cord Cell Therapy Improves Spatial Memory in Aging Rats

There is a growing interest in the potential of adult stem cells for implementing regenerative medicine in the brain. We assessed the effect of intracerebroventricular (icv) administration of human umbilical cord perivascular cells (HUCPVCs) on spatial memory of senile (27 mo) female rats, using int...

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Detalles Bibliográficos
Autores principales: Lehmann, Marianne, Zappa Villar, María Florencia, Garcia, Mariana, Mazzolini, Guillermo, Canatelli Mallat, Martina, Morel, Gustavo Ramón, Reggiani, Paula Cecilia, Goya, Rodolfo Gustavo
Formato: Articulo
Lenguaje:Inglés
Publicado: 2019
Materias:
Medicina
Biología
Brain aging
Spatial memory
Hippocampus
Umbilical cord
Stem cells
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/142072
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-142072
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Medicina
Biología
Brain aging
Spatial memory
Hippocampus
Umbilical cord
Stem cells
spellingShingle Medicina
Biología
Brain aging
Spatial memory
Hippocampus
Umbilical cord
Stem cells
Lehmann, Marianne
Zappa Villar, María Florencia
Garcia, Mariana
Mazzolini, Guillermo
Canatelli Mallat, Martina
Morel, Gustavo Ramón
Reggiani, Paula Cecilia
Goya, Rodolfo Gustavo
Umbilical Cord Cell Therapy Improves Spatial Memory in Aging Rats
topic_facet Medicina
Biología
Brain aging
Spatial memory
Hippocampus
Umbilical cord
Stem cells
description There is a growing interest in the potential of adult stem cells for implementing regenerative medicine in the brain. We assessed the effect of intracerebroventricular (icv) administration of human umbilical cord perivascular cells (HUCPVCs) on spatial memory of senile (27 mo) female rats, using intact senile counterparts as controls. Approximately one third of the animals were injected in the lateral ventricles with a suspension containing 4.8 X 105 HUCPVC in 8 μl per side. The other third received 4.8 X 105 transgenic HUCPVC overexpressing Insulin-like growth factor-1 (IGF-1) and the last third of the rats received no treatment. Spatial memory performance was evaluated using a modified version of the Barnes maze test. In order to evaluate learning ability as well as spatial memory retention, we assessed the time spent (permanence) by animals in goal sector 1 (GS1) and 3 (GS3) when the escape box was removed. Fluorescence microscopy revealed the prescence of Dil-labeled HUCPVC in coronal sections of treated brains. The HUCPVC were located in close contact with the ependymal cells with only a few labeled cells migrating into the brain parenchyma. After treatment with naive or IGF-1 transgenic HUCPVC, permanence in GS1 and GS3 increased significantly whereas there were no changes in the intact animals. We conclude that HUCPVC injected icv are effective to improve some components of spatial memory in senile rats. The ready accessibility of HUCPVC constitutes a significant incentive to continue the exploration of their therapeutic potential on neurodegenerative diseases.
format Articulo
Articulo
author Lehmann, Marianne
Zappa Villar, María Florencia
Garcia, Mariana
Mazzolini, Guillermo
Canatelli Mallat, Martina
Morel, Gustavo Ramón
Reggiani, Paula Cecilia
Goya, Rodolfo Gustavo
author_facet Lehmann, Marianne
Zappa Villar, María Florencia
Garcia, Mariana
Mazzolini, Guillermo
Canatelli Mallat, Martina
Morel, Gustavo Ramón
Reggiani, Paula Cecilia
Goya, Rodolfo Gustavo
author_sort Lehmann, Marianne
title Umbilical Cord Cell Therapy Improves Spatial Memory in Aging Rats
title_short Umbilical Cord Cell Therapy Improves Spatial Memory in Aging Rats
title_full Umbilical Cord Cell Therapy Improves Spatial Memory in Aging Rats
title_fullStr Umbilical Cord Cell Therapy Improves Spatial Memory in Aging Rats
title_full_unstemmed Umbilical Cord Cell Therapy Improves Spatial Memory in Aging Rats
title_sort umbilical cord cell therapy improves spatial memory in aging rats
publishDate 2019
url http://sedici.unlp.edu.ar/handle/10915/142072
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