An alternative method in distinguishing cattle transferrin phenotypes

A modification of the method described by Kristjansson (1963) allows easier distinction of the components and position of every major cattle transferrin phenotype. The modification is based on increasing the percentage of starch (15%) and reducing the pH of the gel buffer to 6.8. In all the experime...

Descripción completa

Detalles Bibliográficos
Autores principales:	Quinteros, Indalecio Rodolfo, Miller, Wilmer J.
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	1968
Materias:	Veterinaria Química cattle transferrin phenotype starch gel buffer electrophoresis
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/142057
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-142057
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Veterinaria Química cattle transferrin phenotype starch gel buffer electrophoresis
spellingShingle	Veterinaria Química cattle transferrin phenotype starch gel buffer electrophoresis Quinteros, Indalecio Rodolfo Miller, Wilmer J. An alternative method in distinguishing cattle transferrin phenotypes
topic_facet	Veterinaria Química cattle transferrin phenotype starch gel buffer electrophoresis
description	A modification of the method described by Kristjansson (1963) allows easier distinction of the components and position of every major cattle transferrin phenotype. The modification is based on increasing the percentage of starch (15%) and reducing the pH of the gel buffer to 6.8. In all the experiments, when a voltage of 350 was applied, a tray of ice was placed over the starch gel for the remainder of the electrophoresis. Different cattle transferrin phenotypes from our modified electrophoresis method are composed as follows: Type A, 4 bands; D1, 4 bands; D1D2, 4 bands; D2, 4 bands; E, 4 bands; AD1, 6 bands; AD2, 6 bands; AE, 8 bands and sometimes 9 bands; D1E, 6 bands and sometimes 7 bands; and D2E, 6 bands. The position of the fourth D band is distinctly different in D1 vs. D2 types.
format	Articulo Articulo
author	Quinteros, Indalecio Rodolfo Miller, Wilmer J.
author_facet	Quinteros, Indalecio Rodolfo Miller, Wilmer J.
author_sort	Quinteros, Indalecio Rodolfo
title	An alternative method in distinguishing cattle transferrin phenotypes
title_short	An alternative method in distinguishing cattle transferrin phenotypes
title_full	An alternative method in distinguishing cattle transferrin phenotypes
title_fullStr	An alternative method in distinguishing cattle transferrin phenotypes
title_full_unstemmed	An alternative method in distinguishing cattle transferrin phenotypes
title_sort	alternative method in distinguishing cattle transferrin phenotypes
publishDate	1968
url	http://sedici.unlp.edu.ar/handle/10915/142057
work_keys_str_mv	AT quinterosindaleciorodolfo analternativemethodindistinguishingcattletransferrinphenotypes AT millerwilmerj analternativemethodindistinguishingcattletransferrinphenotypes AT quinterosindaleciorodolfo alternativemethodindistinguishingcattletransferrinphenotypes AT millerwilmerj alternativemethodindistinguishingcattletransferrinphenotypes
bdutipo_str	Repositorios
_version_	1764820458689003521

An alternative method in distinguishing cattle transferrin phenotypes

Ejemplares similares