An Enzymatic–Colorimetric Assay for the Quantification of <i>Bifidobacterium</i>

An enzymatic-colorimetric assay for the quantification of <i>Bifidobacterium</i> was developed. The method, based upon the standard detection of fructose-6-phosphate phosphoketolase activity, was optimized with respect to bacterial cell pretreatment, time of incubation, and substrate con...

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Detalles Bibliográficos
Autores principales: Bibiloni, Rodrigo, Pérez, Pablo Fernando, De Antoni, Graciela Liliana
Formato: Articulo
Lenguaje:Inglés
Publicado: 2000
Materias:
Ciencias Exactas
Química
Bifidobacterium
enzymatic-colorimetric assay
Bacteria
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/141899
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-141899
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Química
Bifidobacterium
enzymatic-colorimetric assay
Bacteria
spellingShingle Ciencias Exactas
Química
Bifidobacterium
enzymatic-colorimetric assay
Bacteria
Bibiloni, Rodrigo
Pérez, Pablo Fernando
De Antoni, Graciela Liliana
An Enzymatic–Colorimetric Assay for the Quantification of <i>Bifidobacterium</i>
topic_facet Ciencias Exactas
Química
Bifidobacterium
enzymatic-colorimetric assay
Bacteria
description An enzymatic-colorimetric assay for the quantification of <i>Bifidobacterium</i> was developed. The method, based upon the standard detection of fructose-6-phosphate phosphoketolase activity, was optimized with respect to bacterial cell pretreatment, time of incubation, and substrate concentration. The relationship between bacterial biomass and phosphoketolase activity was linear in a wide spectrum of bacterial densities. Higher sensitivity over the standard method was achieved by using 0.25% Triton X-100 in the reaction mixture to pretreat the bacterial cells. Because autoaggregation is a frequent feature among <i>Bifidobacterium</i> strains, this simple and reproducible method offers good advantage over viable plate count and turbidimetric techniques. The methodology can also be applied to the assessment of adherent <i>Bifidobacterium</i> strains to human epithelial cells.
format Articulo
Articulo
author Bibiloni, Rodrigo
Pérez, Pablo Fernando
De Antoni, Graciela Liliana
author_facet Bibiloni, Rodrigo
Pérez, Pablo Fernando
De Antoni, Graciela Liliana
author_sort Bibiloni, Rodrigo
title An Enzymatic–Colorimetric Assay for the Quantification of <i>Bifidobacterium</i>
title_short An Enzymatic–Colorimetric Assay for the Quantification of <i>Bifidobacterium</i>
title_full An Enzymatic–Colorimetric Assay for the Quantification of <i>Bifidobacterium</i>
title_fullStr An Enzymatic–Colorimetric Assay for the Quantification of <i>Bifidobacterium</i>
title_full_unstemmed An Enzymatic–Colorimetric Assay for the Quantification of <i>Bifidobacterium</i>
title_sort enzymatic–colorimetric assay for the quantification of <i>bifidobacterium</i>
publishDate 2000
url http://sedici.unlp.edu.ar/handle/10915/141899
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