Sodium Selenite Improves In Vitro Maturation of Bos primigenius taurus Oocytes

Selenium (Se) is an essential trace element with important functions in animals and whose deficiency is associated with reproductive failures. The aim of this study was to investigate the effect of Se concentrations during in vitro maturation (IVM) of Bos taurus oocyte within the reference ranges fo...

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Autores principales: Lizarraga, Raúl Martín, Anchordoquy, Juan Mateo, Galarza, Esteban Martín, Farnetano, Nicolás Agustín, Carranza Martín, Ana Cristina, Furnus, Cecilia Cristina, Mattioli, Guillermo Alberto, Anchordoquy, Juan Patricio
Formato: Articulo
Lenguaje:Inglés
Publicado: 2019
Materias:
Veterinaria
Selenium
MDA
TAC
Apoptosis
GSH
Embryo development
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/141479
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-141479
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Veterinaria
Selenium
MDA
TAC
Apoptosis
GSH
Embryo development
spellingShingle Veterinaria
Selenium
MDA
TAC
Apoptosis
GSH
Embryo development
Lizarraga, Raúl Martín
Anchordoquy, Juan Mateo
Galarza, Esteban Martín
Farnetano, Nicolás Agustín
Carranza Martín, Ana Cristina
Furnus, Cecilia Cristina
Mattioli, Guillermo Alberto
Anchordoquy, Juan Patricio
Sodium Selenite Improves In Vitro Maturation of Bos primigenius taurus Oocytes
topic_facet Veterinaria
Selenium
MDA
TAC
Apoptosis
GSH
Embryo development
description Selenium (Se) is an essential trace element with important functions in animals and whose deficiency is associated with reproductive failures. The aim of this study was to investigate the effect of Se concentrations during in vitro maturation (IVM) of Bos taurus oocyte within the reference ranges for Se status in cattle. For this purpose, Aberdeen Angus cumulus–oocyte complexes (COCs) were matured in IVM medium supplemented with 0, 10, 50, and 100 ng/mL Se (control, deficient, marginal, and adequate, respectively). The results demonstrated that marginal and adequate Se concentrations added during IVM increased viability and non-apoptotic cumulus cells (CC). Moreover, the addition of Se to culture media decreased malondialdehyde level in COC with all studied concentrations and increased total glutathione content in CC and oocytes with 10 ng/mL Se. On the other hand, total antioxidant capacity of COC, nuclear maturation, and the developmental capacity of oocytes were not modified by Se supplementation. However, 10 ng/mL Se increased hatching rate. In conclusion, supplementation with 10 ng/mL Se during in vitro maturation of Bos primigenius taurus oocytes should be considered to improve embryo quality.
format Articulo
Articulo
author Lizarraga, Raúl Martín
Anchordoquy, Juan Mateo
Galarza, Esteban Martín
Farnetano, Nicolás Agustín
Carranza Martín, Ana Cristina
Furnus, Cecilia Cristina
Mattioli, Guillermo Alberto
Anchordoquy, Juan Patricio
author_facet Lizarraga, Raúl Martín
Anchordoquy, Juan Mateo
Galarza, Esteban Martín
Farnetano, Nicolás Agustín
Carranza Martín, Ana Cristina
Furnus, Cecilia Cristina
Mattioli, Guillermo Alberto
Anchordoquy, Juan Patricio
author_sort Lizarraga, Raúl Martín
title Sodium Selenite Improves In Vitro Maturation of Bos primigenius taurus Oocytes
title_short Sodium Selenite Improves In Vitro Maturation of Bos primigenius taurus Oocytes
title_full Sodium Selenite Improves In Vitro Maturation of Bos primigenius taurus Oocytes
title_fullStr Sodium Selenite Improves In Vitro Maturation of Bos primigenius taurus Oocytes
title_full_unstemmed Sodium Selenite Improves In Vitro Maturation of Bos primigenius taurus Oocytes
title_sort sodium selenite improves in vitro maturation of bos primigenius taurus oocytes
publishDate 2019
url http://sedici.unlp.edu.ar/handle/10915/141479
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