Fluorescein Diacetate Hydrolysis as a Measure of Fungal Biomass in Soil
The fatty acid methyl esters of lipids extracted from an agricultural soil in the preharvest period of soybean or middle growth cycle from wheat were characterized and quantified by gas-liquid chromatography. The fatty acids 18:2ω6 and 16:1ω5 were used as markers of saprotrophic and arbuscular mycor...
Guardado en:
| Autores principales: | , , , |
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| Formato: | Articulo |
| Lenguaje: | Inglés |
| Publicado: |
2001
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| Materias: | |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/141463 |
| Aporte de: |
| id |
I19-R120-10915-141463 |
|---|---|
| record_format |
dspace |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Biología Ciencias Naturales Biomass Methyl Ester Microbial Biomass Arbuscular Mycorrhizal Fungus Nitrogen Content |
| spellingShingle |
Biología Ciencias Naturales Biomass Methyl Ester Microbial Biomass Arbuscular Mycorrhizal Fungus Nitrogen Content Gaspar, María Laura Cabello, Marta Noemí Pollero, Ricardo José Aon, Miguel A. Fluorescein Diacetate Hydrolysis as a Measure of Fungal Biomass in Soil |
| topic_facet |
Biología Ciencias Naturales Biomass Methyl Ester Microbial Biomass Arbuscular Mycorrhizal Fungus Nitrogen Content |
| description |
The fatty acid methyl esters of lipids extracted from an agricultural soil in the preharvest period of soybean or middle growth cycle from wheat were characterized and quantified by gas-liquid chromatography. The fatty acids 18:2ω6 and 16:1ω5 were used as markers of saprotrophic and arbuscular mycorrhizal fungi. In parallel, biomass estimation through plate counts in selective media for cellulolytic and saprotrophic fungi was also performed all throughout a soybean crop or middle growth cycle of wheat. As an enzymatic method, the fluorescein diacetate (FDA) hydrolytic activity of the samples was determined. Owing to the high relationship exhibited by FDA hydrolysis with organic carbon and total nitrogen content of soil, the enzymatic activity was correlated with the microbial biomass estimated through marker lipids or plate counts. The results obtained point out that FDA hydrolysis may be used as a rapid, cheap, and reliable estimator of fungal biomass. |
| format |
Articulo Articulo |
| author |
Gaspar, María Laura Cabello, Marta Noemí Pollero, Ricardo José Aon, Miguel A. |
| author_facet |
Gaspar, María Laura Cabello, Marta Noemí Pollero, Ricardo José Aon, Miguel A. |
| author_sort |
Gaspar, María Laura |
| title |
Fluorescein Diacetate Hydrolysis as a Measure of Fungal Biomass in Soil |
| title_short |
Fluorescein Diacetate Hydrolysis as a Measure of Fungal Biomass in Soil |
| title_full |
Fluorescein Diacetate Hydrolysis as a Measure of Fungal Biomass in Soil |
| title_fullStr |
Fluorescein Diacetate Hydrolysis as a Measure of Fungal Biomass in Soil |
| title_full_unstemmed |
Fluorescein Diacetate Hydrolysis as a Measure of Fungal Biomass in Soil |
| title_sort |
fluorescein diacetate hydrolysis as a measure of fungal biomass in soil |
| publishDate |
2001 |
| url |
http://sedici.unlp.edu.ar/handle/10915/141463 |
| work_keys_str_mv |
AT gasparmarialaura fluoresceindiacetatehydrolysisasameasureoffungalbiomassinsoil AT cabellomartanoemi fluoresceindiacetatehydrolysisasameasureoffungalbiomassinsoil AT polleroricardojose fluoresceindiacetatehydrolysisasameasureoffungalbiomassinsoil AT aonmiguela fluoresceindiacetatehydrolysisasameasureoffungalbiomassinsoil |
| bdutipo_str |
Repositorios |
| _version_ |
1764820459116822530 |