Effect of different carbon sources on Δ5 desaturation activity of HTC cells

The effect of different carbon sources on Δ5 desaturation activity in Minimal Deviation Hepatoma 7288 c (HTC) cells was studied. When HTC cells were incubated in the absence of carbon sources in the medium (fasted cells) the conversion of [1-14C]eicosa-8,11,14-trienoic acid to arachidonic acid incre...

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Detalles Bibliográficos
Autores principales: Tacconi de Alaniz, María Josefa, Tacconi de Gómez Dumm, Irma Nelva, Brenner, Rodolfo Roberto
Formato: Articulo
Lenguaje:Inglés
Publicado: 1982
Materias:
Ciencias Exactas
Δ5 desaturation
Minimal Deviation Hepatoma 7288 c
Fisiología
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/141342
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-141342
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Δ5 desaturation
Minimal Deviation Hepatoma 7288 c
Fisiología
spellingShingle Ciencias Exactas
Δ5 desaturation
Minimal Deviation Hepatoma 7288 c
Fisiología
Tacconi de Alaniz, María Josefa
Tacconi de Gómez Dumm, Irma Nelva
Brenner, Rodolfo Roberto
Effect of different carbon sources on Δ5 desaturation activity of HTC cells
topic_facet Ciencias Exactas
Δ5 desaturation
Minimal Deviation Hepatoma 7288 c
Fisiología
description The effect of different carbon sources on Δ5 desaturation activity in Minimal Deviation Hepatoma 7288 c (HTC) cells was studied. When HTC cells were incubated in the absence of carbon sources in the medium (fasted cells) the conversion of [1-14C]eicosa-8,11,14-trienoic acid to arachidonic acid increased significantly compared to those cells incubated in S-77 medium (fed cells). This activity was not modified if fasted. cells were refed with glucose. However, the activity decreased significantly by the addition of lactalbumin hydrolysate to fasted cells. This effect can not be attributed to individual aminoacids since the addition of them to an aminoacid free medium produced no changes on Δ5 desaturation activity. The incubation of HTC cells with S-77 medium supplemented with glucose, pyruvate or glucose plus pyruvate, showed similar Δ5 desaturation activity to those cells incubated in S-77 medium alone.
format Articulo
Articulo
author Tacconi de Alaniz, María Josefa
Tacconi de Gómez Dumm, Irma Nelva
Brenner, Rodolfo Roberto
author_facet Tacconi de Alaniz, María Josefa
Tacconi de Gómez Dumm, Irma Nelva
Brenner, Rodolfo Roberto
author_sort Tacconi de Alaniz, María Josefa
title Effect of different carbon sources on Δ5 desaturation activity of HTC cells
title_short Effect of different carbon sources on Δ5 desaturation activity of HTC cells
title_full Effect of different carbon sources on Δ5 desaturation activity of HTC cells
title_fullStr Effect of different carbon sources on Δ5 desaturation activity of HTC cells
title_full_unstemmed Effect of different carbon sources on Δ5 desaturation activity of HTC cells
title_sort effect of different carbon sources on δ5 desaturation activity of htc cells
publishDate 1982
url http://sedici.unlp.edu.ar/handle/10915/141342
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AT tacconidegomezdummirmanelva effectofdifferentcarbonsourcesond5desaturationactivityofhtccells
AT brennerrodolforoberto effectofdifferentcarbonsourcesond5desaturationactivityofhtccells
bdutipo_str Repositorios
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