A derivative UV spectrophotometric method for the determination of levothyroxine sodium in tablets

A derivative UV (D-UV) spectrophotometric method was developed for the determination of Levothyroxine Sodium (L-T4) in tablets of different doses. Quantification was performed using the second derivative of the absorption spectrum at 253 nm (2D253) in methanol: water (50: 50; v/v) (pH 11.2). The met...

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Detalles Bibliográficos
Autores principales: Gregorini, Anabella, Ruiz, María Esperanza, Volonté, María Guillermina
Formato: Articulo
Lenguaje:Inglés
Publicado: 2013
Materias:
Ciencias Exactas
levothyroxine sodium
derivative UV spectrophotometry
high performance liquid chromatography
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/141316
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-141316
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
levothyroxine sodium
derivative UV spectrophotometry
high performance liquid chromatography
spellingShingle Ciencias Exactas
levothyroxine sodium
derivative UV spectrophotometry
high performance liquid chromatography
Gregorini, Anabella
Ruiz, María Esperanza
Volonté, María Guillermina
A derivative UV spectrophotometric method for the determination of levothyroxine sodium in tablets
topic_facet Ciencias Exactas
levothyroxine sodium
derivative UV spectrophotometry
high performance liquid chromatography
description A derivative UV (D-UV) spectrophotometric method was developed for the determination of Levothyroxine Sodium (L-T4) in tablets of different doses. Quantification was performed using the second derivative of the absorption spectrum at 253 nm (2D253) in methanol: water (50: 50; v/v) (pH 11.2). The method was validated and compared with an HPLC procedure carried out using a RP-18 column (125 × 4 mm, 5 μm) and methanol: phosphoric acid (0.1%) (70: 30, v/v) (pH 3) as mobile phase. Flow rate was set at 1.5 mL/min, and detection was performed at 225 nm. The proposed D-UV method was linear in the range 3.0–40.0 μg/mL with an appropriate precision and accuracy, and it was selective for the drug under study. On the other hand, results obtained by 2D253 analysis were similar to those obtained by HPLC, with no statistically significant differences between them. Therefore, it was concluded that the developed method is suitable for the determination of L-T4 in tablets at the tested doses.
format Articulo
Articulo
author Gregorini, Anabella
Ruiz, María Esperanza
Volonté, María Guillermina
author_facet Gregorini, Anabella
Ruiz, María Esperanza
Volonté, María Guillermina
author_sort Gregorini, Anabella
title A derivative UV spectrophotometric method for the determination of levothyroxine sodium in tablets
title_short A derivative UV spectrophotometric method for the determination of levothyroxine sodium in tablets
title_full A derivative UV spectrophotometric method for the determination of levothyroxine sodium in tablets
title_fullStr A derivative UV spectrophotometric method for the determination of levothyroxine sodium in tablets
title_full_unstemmed A derivative UV spectrophotometric method for the determination of levothyroxine sodium in tablets
title_sort derivative uv spectrophotometric method for the determination of levothyroxine sodium in tablets
publishDate 2013
url http://sedici.unlp.edu.ar/handle/10915/141316
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