Differential Outcome between BALB/c and C57BL/6 Mice after <i>Escherichia coli</i> O157:H7 Infection Is Associated with a Dissimilar Tolerance Mechanism
Enterohemorrhagic <i>Escherichia coli</i> (EHEC) infections can result in a wide range of clinical presentations despite that EHEC strains belong to the O157:H7 serotype, one of the most pathogenic forms. Although pathogen virulence influences disease outcome, we emphasize the concept of...
| Autores principales: | , , , , , , , , , |
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| Formato: | Articulo |
| Lenguaje: | Inglés |
| Publicado: |
2021
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| Materias: | |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/141078 |
| Aporte de: |
| id |
I19-R120-10915-141078 |
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| record_format |
dspace |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Medicina Ciencias Exactas EHEC intestinal infection mouse model defense mechanisms antibodies Shiga toxins |
| spellingShingle |
Medicina Ciencias Exactas EHEC intestinal infection mouse model defense mechanisms antibodies Shiga toxins Bernal, Alan Mauro Fernandez-Brando, Romina Jimena Bruballa, Andrea Cecilia Fiorentino, Gabriela A. Pineda, Gonzalo Ezequiel Zotta, Elsa Vermeulen, Mónica Ramos, María Victoria Rumbo, Martín Palermo, Marina Sandra Differential Outcome between BALB/c and C57BL/6 Mice after <i>Escherichia coli</i> O157:H7 Infection Is Associated with a Dissimilar Tolerance Mechanism |
| topic_facet |
Medicina Ciencias Exactas EHEC intestinal infection mouse model defense mechanisms antibodies Shiga toxins |
| description |
Enterohemorrhagic <i>Escherichia coli</i> (EHEC) infections can result in a wide range of clinical presentations despite that EHEC strains belong to the O157:H7 serotype, one of the most pathogenic forms. Although pathogen virulence influences disease outcome, we emphasize the concept of host-pathogen interactions, which involve resistance or tolerance mechanisms in the host that determine total host fitness and bacterial virulence. Taking advantage of the genetic differences between mouse strains, we analyzed the clinical progression in C57BL/6 and BALB/c weaned mice infected with an <i>E. coli</i> O157:H7 strain. We carefully analyzed colonization with several bacterial doses, clinical parameters, intestinal histology, and the integrity of the intestinal barrier, as well as local and systemic levels of antibodies to pathogenic factors. We demonstrated that although both strains had comparable susceptibility to Shiga toxin (Stx) and the intestinal bacterial burden was similar, C57BL/6 showed increased intestinal damage, alteration of the integrity of the intestinal barrier, and impaired renal function that resulted in increased mortality. The increased survival rate in the BALB/c strain was associated with an early specific antibody response as part of a tolerance mechanism. |
| format |
Articulo Articulo |
| author |
Bernal, Alan Mauro Fernandez-Brando, Romina Jimena Bruballa, Andrea Cecilia Fiorentino, Gabriela A. Pineda, Gonzalo Ezequiel Zotta, Elsa Vermeulen, Mónica Ramos, María Victoria Rumbo, Martín Palermo, Marina Sandra |
| author_facet |
Bernal, Alan Mauro Fernandez-Brando, Romina Jimena Bruballa, Andrea Cecilia Fiorentino, Gabriela A. Pineda, Gonzalo Ezequiel Zotta, Elsa Vermeulen, Mónica Ramos, María Victoria Rumbo, Martín Palermo, Marina Sandra |
| author_sort |
Bernal, Alan Mauro |
| title |
Differential Outcome between BALB/c and C57BL/6 Mice after <i>Escherichia coli</i> O157:H7 Infection Is Associated with a Dissimilar Tolerance Mechanism |
| title_short |
Differential Outcome between BALB/c and C57BL/6 Mice after <i>Escherichia coli</i> O157:H7 Infection Is Associated with a Dissimilar Tolerance Mechanism |
| title_full |
Differential Outcome between BALB/c and C57BL/6 Mice after <i>Escherichia coli</i> O157:H7 Infection Is Associated with a Dissimilar Tolerance Mechanism |
| title_fullStr |
Differential Outcome between BALB/c and C57BL/6 Mice after <i>Escherichia coli</i> O157:H7 Infection Is Associated with a Dissimilar Tolerance Mechanism |
| title_full_unstemmed |
Differential Outcome between BALB/c and C57BL/6 Mice after <i>Escherichia coli</i> O157:H7 Infection Is Associated with a Dissimilar Tolerance Mechanism |
| title_sort |
differential outcome between balb/c and c57bl/6 mice after <i>escherichia coli</i> o157:h7 infection is associated with a dissimilar tolerance mechanism |
| publishDate |
2021 |
| url |
http://sedici.unlp.edu.ar/handle/10915/141078 |
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Repositorios |
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