Rhomboid family gene expression profiling in breast normal tissue and tumor samples

Rhomboid is an evolutionary conserved and functionally diversified group of proteins composed of proteolytically active and inactive members that are involved in the modulation of multiple biological processes such as epidermal growth factor receptor signaling pathway, endoplasmic reticulum-associat...

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Autores principales: Canzoneri, Romina, Lacunza, Ezequiel, Isla Larrain, Marina Teresita, Croce, María Virginia, Abba, Martín Carlos
Formato: Articulo
Lenguaje:Inglés
Publicado: 2014
Materias:
Medicina
Rhomboid
Gene expression
Breast cancer
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/141032
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-141032
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Medicina
Rhomboid
Gene expression
Breast cancer
spellingShingle Medicina
Rhomboid
Gene expression
Breast cancer
Canzoneri, Romina
Lacunza, Ezequiel
Isla Larrain, Marina Teresita
Croce, María Virginia
Abba, Martín Carlos
Rhomboid family gene expression profiling in breast normal tissue and tumor samples
topic_facet Medicina
Rhomboid
Gene expression
Breast cancer
description Rhomboid is an evolutionary conserved and functionally diversified group of proteins composed of proteolytically active and inactive members that are involved in the modulation of multiple biological processes such as epidermal growth factor receptor signaling pathway, endoplasmic reticulum-associated degradation, cell death, and proliferation. Recently, several human rhomboid genes have been associated with the development of chronic myeloid leukemia and pituitary, colorectal, ovarian, and breast cancers. In this study, we evaluated the mRNA and protein expression profiles of rhomboid genes in cancer cell lines and breast tissue/tumor samples. In silico analysis of publicly available gene expression datasets showed that different rhomboid genes are specifically expressed according to the breast cancer intrinsic subtypes. Quantitative reverse transcription–polymerase chain reaction (RT-PCR) analysis showed a significant RHBDD2 mRNA overexpression in advanced breast cancer compared with normal tissue samples (p = 0.012). In addition, we found that RHBDL2 and PARL mRNA expression was associated with a low/intermediate histologic tumor grade (p = 0.024 and p = 0.015, respectively). Immunohistochemistry analysis showed a significant increase of RHBDD2 protein expression in association with breast cancer samples negative for progesterone receptor (p = 0.015). Moreover, protein expression analysis corroborated the quantitative RT-PCR results, indicating that breast primary tumors belonging to patients with a more disseminated disease expressed significantly increased levels of RHBDD2 protein compared with less disseminated tumors (p = 0.01).
format Articulo
Articulo
author Canzoneri, Romina
Lacunza, Ezequiel
Isla Larrain, Marina Teresita
Croce, María Virginia
Abba, Martín Carlos
author_facet Canzoneri, Romina
Lacunza, Ezequiel
Isla Larrain, Marina Teresita
Croce, María Virginia
Abba, Martín Carlos
author_sort Canzoneri, Romina
title Rhomboid family gene expression profiling in breast normal tissue and tumor samples
title_short Rhomboid family gene expression profiling in breast normal tissue and tumor samples
title_full Rhomboid family gene expression profiling in breast normal tissue and tumor samples
title_fullStr Rhomboid family gene expression profiling in breast normal tissue and tumor samples
title_full_unstemmed Rhomboid family gene expression profiling in breast normal tissue and tumor samples
title_sort rhomboid family gene expression profiling in breast normal tissue and tumor samples
publishDate 2014
url http://sedici.unlp.edu.ar/handle/10915/141032
work_keys_str_mv AT canzoneriromina rhomboidfamilygeneexpressionprofilinginbreastnormaltissueandtumorsamples
AT lacunzaezequiel rhomboidfamilygeneexpressionprofilinginbreastnormaltissueandtumorsamples
AT islalarrainmarinateresita rhomboidfamilygeneexpressionprofilinginbreastnormaltissueandtumorsamples
AT crocemariavirginia rhomboidfamilygeneexpressionprofilinginbreastnormaltissueandtumorsamples
AT abbamartincarlos rhomboidfamilygeneexpressionprofilinginbreastnormaltissueandtumorsamples
bdutipo_str Repositorios
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