Insights into Hydrocarbon Assimilation by Eurotialean and Hypocrealean Fungi : Roles for CYP52 and CYP53 Clans of Cytochrome P450 Genes

Several filamentous fungi are able to concomitantly assimilate both aliphatic and polycyclic aromatic hydrocarbons that are the biogenic by-products of some industrial processes. Cytochrome P450 monooxygenases catalyze the first oxidation reaction for both types of substrate. Among the cytochrome P4...

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Autores principales: Huarte Bonnet, Carla, Kumar, Suresh, Saparrat, Mario Carlos Nazareno, Girotti, Juan Roberto, Santana, Marianela, Hallsworth, John E., Pedrini, Nicolás
Formato: Articulo
Lenguaje:Inglés
Publicado: 2017
Materias:
Biología
Ciencias Naturales
Aspergillus niger
Beauveria bassiana
Entomopathogenic fungi
Hydrocarbon degradation
Metarhizium anisopliae
Penicillium chrysogenum
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/141006
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-141006
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Ciencias Naturales
Aspergillus niger
Beauveria bassiana
Entomopathogenic fungi
Hydrocarbon degradation
Metarhizium anisopliae
Penicillium chrysogenum
spellingShingle Biología
Ciencias Naturales
Aspergillus niger
Beauveria bassiana
Entomopathogenic fungi
Hydrocarbon degradation
Metarhizium anisopliae
Penicillium chrysogenum
Huarte Bonnet, Carla
Kumar, Suresh
Saparrat, Mario Carlos Nazareno
Girotti, Juan Roberto
Santana, Marianela
Hallsworth, John E.
Pedrini, Nicolás
Insights into Hydrocarbon Assimilation by Eurotialean and Hypocrealean Fungi : Roles for CYP52 and CYP53 Clans of Cytochrome P450 Genes
topic_facet Biología
Ciencias Naturales
Aspergillus niger
Beauveria bassiana
Entomopathogenic fungi
Hydrocarbon degradation
Metarhizium anisopliae
Penicillium chrysogenum
description Several filamentous fungi are able to concomitantly assimilate both aliphatic and polycyclic aromatic hydrocarbons that are the biogenic by-products of some industrial processes. Cytochrome P450 monooxygenases catalyze the first oxidation reaction for both types of substrate. Among the cytochrome P450 (CYP) genes, the family CYP52 is implicated in the first hydroxylation step in alkane-assimilation processes, while genes belonging to the family CYP53 have been linked with oxidation of aromatic hydrocarbons. Here, we perform a comparative analysis of CYP genes belonging to clans CYP52 and CYP53 in Aspergillus niger, Beauveria bassiana, Metarhizium robertsii (formerly M. anisopliae var. anisopliae), and Penicillium chrysogenum. These species were able to assimilate n-hexadecane, n-octacosane, and phenanthrene, exhibiting a species-dependent modification in pH of the nutrient medium during this process. Modeling of the molecular docking of the hydrocarbons to the cytochrome P450 active site revealed that both phenanthrene and n-octacosane are energetically favored as substrates for the enzymes codified by genes belonging to both CYP52 and CYP53 clans, and thus appear to be involved in this oxidation step. Analyses of gene expression revealed that CYP53 members were significantly induced by phenanthrene in all species studied, but only CYP52X1 and CYP53A11 from B. bassiana were highly induced with n-alkanes. These findings suggest that the set of P450 enzymes involved in hydrocarbon assimilation by fungi is dependent on phylogeny and reveal distinct substrate and expression specificities.
format Articulo
Articulo
author Huarte Bonnet, Carla
Kumar, Suresh
Saparrat, Mario Carlos Nazareno
Girotti, Juan Roberto
Santana, Marianela
Hallsworth, John E.
Pedrini, Nicolás
author_facet Huarte Bonnet, Carla
Kumar, Suresh
Saparrat, Mario Carlos Nazareno
Girotti, Juan Roberto
Santana, Marianela
Hallsworth, John E.
Pedrini, Nicolás
author_sort Huarte Bonnet, Carla
title Insights into Hydrocarbon Assimilation by Eurotialean and Hypocrealean Fungi : Roles for CYP52 and CYP53 Clans of Cytochrome P450 Genes
title_short Insights into Hydrocarbon Assimilation by Eurotialean and Hypocrealean Fungi : Roles for CYP52 and CYP53 Clans of Cytochrome P450 Genes
title_full Insights into Hydrocarbon Assimilation by Eurotialean and Hypocrealean Fungi : Roles for CYP52 and CYP53 Clans of Cytochrome P450 Genes
title_fullStr Insights into Hydrocarbon Assimilation by Eurotialean and Hypocrealean Fungi : Roles for CYP52 and CYP53 Clans of Cytochrome P450 Genes
title_full_unstemmed Insights into Hydrocarbon Assimilation by Eurotialean and Hypocrealean Fungi : Roles for CYP52 and CYP53 Clans of Cytochrome P450 Genes
title_sort insights into hydrocarbon assimilation by eurotialean and hypocrealean fungi : roles for cyp52 and cyp53 clans of cytochrome p450 genes
publishDate 2017
url http://sedici.unlp.edu.ar/handle/10915/141006
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