Optimizing Production of Extracellular Laccase from Grammothele Subargentea CLPS No. 436 Strain

The production of extracellular laccase by the Grammothele subargentea CLPS no. 436 strain in liquid cultures grown on a carbon-limited basal medium was significantly enhanced when culture conditions, including the addition of CuSO4·5H2O or veratryl alcohol, were consecutively optimized. A laccase a...

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Autor principal:	Saparrat, Mario Carlos Nazareno
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2004
Materias:	Ciencias Naturales Biología Copper culture agitation extracellular ligninolytic enzymes Grammothele subargentea laccase veratryl alcohol white-rot fungi
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/140947
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-140947
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Ciencias Naturales Biología Copper culture agitation extracellular ligninolytic enzymes Grammothele subargentea laccase veratryl alcohol white-rot fungi
spellingShingle	Ciencias Naturales Biología Copper culture agitation extracellular ligninolytic enzymes Grammothele subargentea laccase veratryl alcohol white-rot fungi Saparrat, Mario Carlos Nazareno Optimizing Production of Extracellular Laccase from Grammothele Subargentea CLPS No. 436 Strain
topic_facet	Ciencias Naturales Biología Copper culture agitation extracellular ligninolytic enzymes Grammothele subargentea laccase veratryl alcohol white-rot fungi
description	The production of extracellular laccase by the Grammothele subargentea CLPS no. 436 strain in liquid cultures grown on a carbon-limited basal medium was significantly enhanced when culture conditions, including the addition of CuSO4·5H2O or veratryl alcohol, were consecutively optimized. A laccase activity as high as 1954.5 mU ml−1 of liquid medium was obtained under optimum conditions, which corresponded to non-agitated cultures supplemented with 0.6 mM CuSO4·5H2O. Veratryl alcohol at 1 mM was less effective than CuSO4·5H2O for increasing laccase activity levels; the supplementation of veratryl alcohol resulted only in maximum levels of 44 mU ml−1 in non-agitated cultures.
format	Articulo Articulo
author	Saparrat, Mario Carlos Nazareno
author_facet	Saparrat, Mario Carlos Nazareno
author_sort	Saparrat, Mario Carlos Nazareno
title	Optimizing Production of Extracellular Laccase from Grammothele Subargentea CLPS No. 436 Strain
title_short	Optimizing Production of Extracellular Laccase from Grammothele Subargentea CLPS No. 436 Strain
title_full	Optimizing Production of Extracellular Laccase from Grammothele Subargentea CLPS No. 436 Strain
title_fullStr	Optimizing Production of Extracellular Laccase from Grammothele Subargentea CLPS No. 436 Strain
title_full_unstemmed	Optimizing Production of Extracellular Laccase from Grammothele Subargentea CLPS No. 436 Strain
title_sort	optimizing production of extracellular laccase from grammothele subargentea clps no. 436 strain
publishDate	2004
url	http://sedici.unlp.edu.ar/handle/10915/140947
work_keys_str_mv	AT saparratmariocarlosnazareno optimizingproductionofextracellularlaccasefromgrammothelesubargenteaclpsno436strain
bdutipo_str	Repositorios
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Optimizing Production of Extracellular Laccase from Grammothele Subargentea CLPS No. 436 Strain

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