Galactomannan as a Potential Modulator of Intestinal Ischemia-Reperfusion Injury

Background: Galactomannan (GAL), a polysaccharide present on the cell wall of several fungi, has shown an ability to modulate inflammatory responses through the dectin-1 receptor in human macrophages. However, studies evaluating the modulatory properties of this polysaccharide in <i>in vivo<...

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Autores principales: Stringa, Pablo Luis, Toledano, Victor, Papa-Gobbi, Rodrigo, Arreola, Miguel, Largo, Carlota, Machuca, Mariana Alejandra, Aguirre, Luis A., Rumbo, Martín, López-Collazo, Eduardo, Hernández Oliveros, Francisco
Formato: Articulo
Lenguaje:Inglés
Publicado: 2020
Materias:
Medicina
Intestine
Reperfusion injury
Mesenteric ischemia
Digestive system surgical procedures
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/140922
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-140922
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Medicina
Intestine
Reperfusion injury
Mesenteric ischemia
Digestive system surgical procedures
spellingShingle Medicina
Intestine
Reperfusion injury
Mesenteric ischemia
Digestive system surgical procedures
Stringa, Pablo Luis
Toledano, Victor
Papa-Gobbi, Rodrigo
Arreola, Miguel
Largo, Carlota
Machuca, Mariana Alejandra
Aguirre, Luis A.
Rumbo, Martín
López-Collazo, Eduardo
Hernández Oliveros, Francisco
Galactomannan as a Potential Modulator of Intestinal Ischemia-Reperfusion Injury
topic_facet Medicina
Intestine
Reperfusion injury
Mesenteric ischemia
Digestive system surgical procedures
description Background: Galactomannan (GAL), a polysaccharide present on the cell wall of several fungi, has shown an ability to modulate inflammatory responses through the dectin-1 receptor in human macrophages. However, studies evaluating the modulatory properties of this polysaccharide in <i>in vivo</i> inflammatory scenarios are scarce. We hypothesized that GAL pretreatment would modulate local and remote damage related to intestinal reperfusion after an ischemic insult. Materials and methods: Adult male Balb/c mice were subjected to intestinal ischemia–reperfusion injury by reversible occlusion of the superior mesenteric artery, consisting of 45 min of ischemia followed by 3 or 24 h of reperfusion. Intragastric GAL (70 mg/kg) was administered 12 h before ischemia, and saline solution was used in the control animals. Jejunum, lung, and blood samples were taken for the analysis of histology, gene expression, plasma cytokine levels, and nitrosative stress. Results: Intestinal and lung histologic alterations were attenuated by GAL pretreatment, showing significant differences compared with nontreated animals. Interleukin 1β, monocyte chemoattractant protein 1, and IL-6 messenger RNA expression were considerably downregulated in the small intestine of the GAL group. In addition, GAL treatment significantly prevented plasma interleukin 6 and monocyte chemoattractant protein 1 upregulation and diminished nitrate and nitrite levels after 3 h of intestinal reperfusion. Conclusions: GAL pretreatment constitutes a novel and promising therapy to reduce local and remote damage triggered by intestinal ischemia–reperfusion injury. Further <i>in vivo</i> and <i>in vitro</i> studies to understand GAL's modulatory effects are warranted.
format Articulo
Articulo
author Stringa, Pablo Luis
Toledano, Victor
Papa-Gobbi, Rodrigo
Arreola, Miguel
Largo, Carlota
Machuca, Mariana Alejandra
Aguirre, Luis A.
Rumbo, Martín
López-Collazo, Eduardo
Hernández Oliveros, Francisco
author_facet Stringa, Pablo Luis
Toledano, Victor
Papa-Gobbi, Rodrigo
Arreola, Miguel
Largo, Carlota
Machuca, Mariana Alejandra
Aguirre, Luis A.
Rumbo, Martín
López-Collazo, Eduardo
Hernández Oliveros, Francisco
author_sort Stringa, Pablo Luis
title Galactomannan as a Potential Modulator of Intestinal Ischemia-Reperfusion Injury
title_short Galactomannan as a Potential Modulator of Intestinal Ischemia-Reperfusion Injury
title_full Galactomannan as a Potential Modulator of Intestinal Ischemia-Reperfusion Injury
title_fullStr Galactomannan as a Potential Modulator of Intestinal Ischemia-Reperfusion Injury
title_full_unstemmed Galactomannan as a Potential Modulator of Intestinal Ischemia-Reperfusion Injury
title_sort galactomannan as a potential modulator of intestinal ischemia-reperfusion injury
publishDate 2020
url http://sedici.unlp.edu.ar/handle/10915/140922
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