Mycobiota on exoskeleton debris of Neohelice granulata in an alkaline-sodic salt marsh : In vitro enzyme ability at different temperatures and pH

This study analysed the mycobiota on exoskeleton debris of the crab Neohelice granulata collected from an alkaline salt marsh and assessed the in vitro enzyme ability of selected isolates at different temperatures and pH. Exoskeleton fragments were incubated in moist chambers on paper and on agar me...

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Autores principales: Ferreri, Natalia Analía, Elíades, Lorena Alejandra, Saparrat, Mario Carlos Nazareno, Ledesma, Tamara Mariel, Russo, María Leticia, Cabello, Marta Noemí
Formato: Articulo
Lenguaje:Inglés
Publicado: 2021
Materias:
Biología
Ciencias Naturales
crab exoskeletons
diversity
enzyme activity
fungal decomposers
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/140874
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-140874
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Ciencias Naturales
crab exoskeletons
diversity
enzyme activity
fungal decomposers
spellingShingle Biología
Ciencias Naturales
crab exoskeletons
diversity
enzyme activity
fungal decomposers
Ferreri, Natalia Analía
Elíades, Lorena Alejandra
Saparrat, Mario Carlos Nazareno
Ledesma, Tamara Mariel
Russo, María Leticia
Cabello, Marta Noemí
Mycobiota on exoskeleton debris of Neohelice granulata in an alkaline-sodic salt marsh : In vitro enzyme ability at different temperatures and pH
topic_facet Biología
Ciencias Naturales
crab exoskeletons
diversity
enzyme activity
fungal decomposers
description This study analysed the mycobiota on exoskeleton debris of the crab Neohelice granulata collected from an alkaline salt marsh and assessed the in vitro enzyme ability of selected isolates at different temperatures and pH. Exoskeleton fragments were incubated in moist chambers on paper and on agar medium. Growth and enzyme ability of selected fungi were also evaluated in agar media with 0.5 % casein, 1% Tween®20, and Chitin-Azure® by the production of a halo/growth ratio. We identified 22 fungal species using both methods. Since the two isolation methods added information to one another, both ones are necessary to recover the cultivable mycobiota associated with the exoskeleton debris. All fungi showed greater levels of enzyme activity in alkaline than acid medium with Tween®20. The halo diameter on casein and chitin varied according to the fungal isolate and pH. Most fungi had a larger halo at 4°C than at the other temperatures tested. Clonostachys rosea showed the greatest activity in all media at 4oC. We conclude that exoskeletons of the N. granulata are a source of fungi able to produce enzyme activities that show differences upon incubation conditions to which they are cultivated such as ones including specific temperatures and pH values.
format Articulo
Articulo
author Ferreri, Natalia Analía
Elíades, Lorena Alejandra
Saparrat, Mario Carlos Nazareno
Ledesma, Tamara Mariel
Russo, María Leticia
Cabello, Marta Noemí
author_facet Ferreri, Natalia Analía
Elíades, Lorena Alejandra
Saparrat, Mario Carlos Nazareno
Ledesma, Tamara Mariel
Russo, María Leticia
Cabello, Marta Noemí
author_sort Ferreri, Natalia Analía
title Mycobiota on exoskeleton debris of Neohelice granulata in an alkaline-sodic salt marsh : In vitro enzyme ability at different temperatures and pH
title_short Mycobiota on exoskeleton debris of Neohelice granulata in an alkaline-sodic salt marsh : In vitro enzyme ability at different temperatures and pH
title_full Mycobiota on exoskeleton debris of Neohelice granulata in an alkaline-sodic salt marsh : In vitro enzyme ability at different temperatures and pH
title_fullStr Mycobiota on exoskeleton debris of Neohelice granulata in an alkaline-sodic salt marsh : In vitro enzyme ability at different temperatures and pH
title_full_unstemmed Mycobiota on exoskeleton debris of Neohelice granulata in an alkaline-sodic salt marsh : In vitro enzyme ability at different temperatures and pH
title_sort mycobiota on exoskeleton debris of neohelice granulata in an alkaline-sodic salt marsh : in vitro enzyme ability at different temperatures and ph
publishDate 2021
url http://sedici.unlp.edu.ar/handle/10915/140874
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