Supplementary material for "RcgA and RcgR, two novel proteins involved in conjugative transfer of rhizobial plasmids"

Rhizobia are gram-negative bacteria able to establish a nitrogen-fixing symbiotic interaction with leguminous plants. Rhizobia usually harbor several plasmids in their genome which can be transferred to other organisms by conjugation. Two main mechanisms of regulation of rhizobial plasmid transfer h...

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Autores principales: Castellani, Lucas Gabriel, Luchetti, Abril, Nilsson, Juliet Fernanda, Pérez Giménez, Julieta, Struck, Ben, Schlüter, Andreas, Pühler, Alfred, Niehaus, Karsten, Romero, David, Pistorio, Mariano, Torres Tejerizo, Gonzalo Arturo
Formato: Conjunto de datos
Lenguaje:Inglés
Publicado: 2022
Materias:
Ciencias Exactas
https://purl.org/becyt/ford/1.6
Rhizobium
plasmids
conjugation
gene regulation
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/140513
https://doi.org/10.35537/10915/140513
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-140513
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
https://purl.org/becyt/ford/1.6
Rhizobium
plasmids
conjugation
gene regulation
spellingShingle Ciencias Exactas
https://purl.org/becyt/ford/1.6
Rhizobium
plasmids
conjugation
gene regulation
Castellani, Lucas Gabriel
Luchetti, Abril
Nilsson, Juliet Fernanda
Pérez Giménez, Julieta
Struck, Ben
Schlüter, Andreas
Pühler, Alfred
Niehaus, Karsten
Romero, David
Pistorio, Mariano
Torres Tejerizo, Gonzalo Arturo
Supplementary material for "RcgA and RcgR, two novel proteins involved in conjugative transfer of rhizobial plasmids"
topic_facet Ciencias Exactas
https://purl.org/becyt/ford/1.6
Rhizobium
plasmids
conjugation
gene regulation
description Rhizobia are gram-negative bacteria able to establish a nitrogen-fixing symbiotic interaction with leguminous plants. Rhizobia usually harbor several plasmids in their genome which can be transferred to other organisms by conjugation. Two main mechanisms of regulation of rhizobial plasmid transfer have been described: Quorum sensing (QS) and rctA/rctB system. Nevertheless, new genes and molecules that modulate conjugative transfer have been recently described, demonstrating that new actors can tightly regulate the process. In this work, by means of bioinformatics tools and molecular biology approaches, two hypothetical genes were identified as playing key roles in conjugative transfer. These genes are located between conjugative genes of plasmid pRfaLPU83a from Rhizobium favelukesii LPU83, a plasmid that shows a conjugative transfer behavior depending on the genomic background. One of the two mentioned genes, rcgA, is essential for conjugation, while the other, rcgR, acts as an inhibitor of the process. In addition to introducing this new regulatory system, we show evidence of the functions of these genes in different genomic backgrounds, and confirmed that homologous proteins from non-closely related organisms have the same function. These findings set up the basis for a new regulatory circuit of conjugative transfer of plasmids.
format Conjunto de datos
Conjunto de datos
author Castellani, Lucas Gabriel
Luchetti, Abril
Nilsson, Juliet Fernanda
Pérez Giménez, Julieta
Struck, Ben
Schlüter, Andreas
Pühler, Alfred
Niehaus, Karsten
Romero, David
Pistorio, Mariano
Torres Tejerizo, Gonzalo Arturo
author_facet Castellani, Lucas Gabriel
Luchetti, Abril
Nilsson, Juliet Fernanda
Pérez Giménez, Julieta
Struck, Ben
Schlüter, Andreas
Pühler, Alfred
Niehaus, Karsten
Romero, David
Pistorio, Mariano
Torres Tejerizo, Gonzalo Arturo
author_sort Castellani, Lucas Gabriel
title Supplementary material for "RcgA and RcgR, two novel proteins involved in conjugative transfer of rhizobial plasmids"
title_short Supplementary material for "RcgA and RcgR, two novel proteins involved in conjugative transfer of rhizobial plasmids"
title_full Supplementary material for "RcgA and RcgR, two novel proteins involved in conjugative transfer of rhizobial plasmids"
title_fullStr Supplementary material for "RcgA and RcgR, two novel proteins involved in conjugative transfer of rhizobial plasmids"
title_full_unstemmed Supplementary material for "RcgA and RcgR, two novel proteins involved in conjugative transfer of rhizobial plasmids"
title_sort supplementary material for "rcga and rcgr, two novel proteins involved in conjugative transfer of rhizobial plasmids"
publishDate 2022
url http://sedici.unlp.edu.ar/handle/10915/140513
https://doi.org/10.35537/10915/140513
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spelling I19-R120-10915-1405132023-04-27T15:09:32Z http://sedici.unlp.edu.ar/handle/10915/140513 https://doi.org/10.35537/10915/140513 Supplementary material for "RcgA and RcgR, two novel proteins involved in conjugative transfer of rhizobial plasmids" Castellani, Lucas Gabriel Luchetti, Abril Nilsson, Juliet Fernanda Pérez Giménez, Julieta Struck, Ben Schlüter, Andreas Pühler, Alfred Niehaus, Karsten Romero, David Pistorio, Mariano Torres Tejerizo, Gonzalo Arturo 2022-08-16T17:25:10Z 2022-08-08 en Ciencias Exactas https://purl.org/becyt/ford/1.6 Rhizobium plasmids conjugation gene regulation Rhizobia are gram-negative bacteria able to establish a nitrogen-fixing symbiotic interaction with leguminous plants. Rhizobia usually harbor several plasmids in their genome which can be transferred to other organisms by conjugation. Two main mechanisms of regulation of rhizobial plasmid transfer have been described: Quorum sensing (QS) and rctA/rctB system. Nevertheless, new genes and molecules that modulate conjugative transfer have been recently described, demonstrating that new actors can tightly regulate the process. In this work, by means of bioinformatics tools and molecular biology approaches, two hypothetical genes were identified as playing key roles in conjugative transfer. These genes are located between conjugative genes of plasmid pRfaLPU83a from Rhizobium favelukesii LPU83, a plasmid that shows a conjugative transfer behavior depending on the genomic background. One of the two mentioned genes, rcgA, is essential for conjugation, while the other, rcgR, acts as an inhibitor of the process. In addition to introducing this new regulatory system, we show evidence of the functions of these genes in different genomic backgrounds, and confirmed that homologous proteins from non-closely related organisms have the same function. These findings set up the basis for a new regulatory circuit of conjugative transfer of plasmids. Fil: Castellani, Lucas Gabriel. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Instituto de Biotecnología y Biología Molecular; Argentina. Fil: Luchetti, Abril. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular; Argentina, Fil: Luchetti, Abril. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina Fil: Nilsson, Juliet Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular; Argentina Fil: Nilsson, Juliet Fernanda. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina Fil: Pérez Giménez, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular; Argentina Fil: Pérez Giménez, Julieta. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina Fil: Pistorio, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular; Argentina Fil: Pistorio, Mariano. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina Fil: Torres Tejerizo, Gonzalo Arturo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular; Argentina Fil: Torres Tejerizo, Gonzalo Arturo. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina Facultad de Ciencias Exactas Consejo Nacional de Investigaciones Científicas y Técnicas Conjunto de datos Conjunto de datos http://creativecommons.org/licenses/by-nc-nd/4.0/ Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) application/pdf

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