Direct detection of human adenovirus or SARS-CoV-2 with ability to inform infectivity using DNA aptamer-nanopore sensors

Viral infections are a major global health issue, but no current method allows rapid, direct, and ultrasensitive quantification of intact viruses with the ability to inform infectivity, causing misdiagnoses and spread of the viruses. Here, we report a method for direct detection and differentiation...

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Autores principales: Peinetti, Ana Sol, Lake, Ryan J., Cong, Wen, Cooper, Laura, Wu, Yuting, Ma, Yuan, Pawel, Gregory T., Toimil-Molares, María Eugenia, Trautmann, Christina, Rong, Lijun, Mariñas, Benito J., Azzaroni, Omar, Lu, Yi
Formato: Articulo
Lenguaje:Inglés
Publicado: 2021
Materias:
Medicina
Ciencias Exactas
direct detection
human adenovirus
SARS-CoV-2
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/140266
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-140266
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Medicina
Ciencias Exactas
direct detection
human adenovirus
SARS-CoV-2
spellingShingle Medicina
Ciencias Exactas
direct detection
human adenovirus
SARS-CoV-2
Peinetti, Ana Sol
Lake, Ryan J.
Cong, Wen
Cooper, Laura
Wu, Yuting
Ma, Yuan
Pawel, Gregory T.
Toimil-Molares, María Eugenia
Trautmann, Christina
Rong, Lijun
Mariñas, Benito J.
Azzaroni, Omar
Lu, Yi
Direct detection of human adenovirus or SARS-CoV-2 with ability to inform infectivity using DNA aptamer-nanopore sensors
topic_facet Medicina
Ciencias Exactas
direct detection
human adenovirus
SARS-CoV-2
description Viral infections are a major global health issue, but no current method allows rapid, direct, and ultrasensitive quantification of intact viruses with the ability to inform infectivity, causing misdiagnoses and spread of the viruses. Here, we report a method for direct detection and differentiation of infectious from noninfectious human adenovirus and SARS-CoV-2, as well as from other virus types, without any sample pretreatment. DNA aptamers are selected from a DNA library to bind intact infectious, but not noninfectious, virus and then incorporated into a solid-state nanopore, which allows strong confinement of the virus to enhance sensitivity down to 1 pfu/ml for human adenovirus and 1 × 10⁴ copies/ml for SARS-CoV-2. Applications of the aptamer-nanopore sensors in different types of water samples, saliva, and serum are demonstrated for both enveloped and nonenveloped viruses, making the sensor generally applicable for detecting these and other emerging viruses of environmental and public health concern.
format Articulo
Articulo
author Peinetti, Ana Sol
Lake, Ryan J.
Cong, Wen
Cooper, Laura
Wu, Yuting
Ma, Yuan
Pawel, Gregory T.
Toimil-Molares, María Eugenia
Trautmann, Christina
Rong, Lijun
Mariñas, Benito J.
Azzaroni, Omar
Lu, Yi
author_facet Peinetti, Ana Sol
Lake, Ryan J.
Cong, Wen
Cooper, Laura
Wu, Yuting
Ma, Yuan
Pawel, Gregory T.
Toimil-Molares, María Eugenia
Trautmann, Christina
Rong, Lijun
Mariñas, Benito J.
Azzaroni, Omar
Lu, Yi
author_sort Peinetti, Ana Sol
title Direct detection of human adenovirus or SARS-CoV-2 with ability to inform infectivity using DNA aptamer-nanopore sensors
title_short Direct detection of human adenovirus or SARS-CoV-2 with ability to inform infectivity using DNA aptamer-nanopore sensors
title_full Direct detection of human adenovirus or SARS-CoV-2 with ability to inform infectivity using DNA aptamer-nanopore sensors
title_fullStr Direct detection of human adenovirus or SARS-CoV-2 with ability to inform infectivity using DNA aptamer-nanopore sensors
title_full_unstemmed Direct detection of human adenovirus or SARS-CoV-2 with ability to inform infectivity using DNA aptamer-nanopore sensors
title_sort direct detection of human adenovirus or sars-cov-2 with ability to inform infectivity using dna aptamer-nanopore sensors
publishDate 2021
url http://sedici.unlp.edu.ar/handle/10915/140266
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