Genetic variants in Argentinean isolates of Spodoptera frugiperda Multiple Nucleopolyhedrovirus

The fall armyworm, Spodoptera frugiperda (JE Smith) is a key pest in the Americas. Control strategies are mainly carried out by use of chemical insecticides and transgenic crops expressing Bacillus thuringiensis toxins. In the last years, resistance of S. frugiperda populations to transgenic corn wa...

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Autores principales: Niz, José María, Salvador, Ricardo, Ferrelli, María Leticia, Sciocco de Cap, Alicia, Romanowski, Víctor, Berretta, Marcelo Facundo
Formato: Articulo
Lenguaje:Inglés
Publicado: 2020
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Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/140243
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id I19-R120-10915-140243
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Biología
Fall armyworm
SfMNPV isolates
Biological control
Baculovirus
Agricultural pest
spellingShingle Ciencias Exactas
Biología
Fall armyworm
SfMNPV isolates
Biological control
Baculovirus
Agricultural pest
Niz, José María
Salvador, Ricardo
Ferrelli, María Leticia
Sciocco de Cap, Alicia
Romanowski, Víctor
Berretta, Marcelo Facundo
Genetic variants in Argentinean isolates of Spodoptera frugiperda Multiple Nucleopolyhedrovirus
topic_facet Ciencias Exactas
Biología
Fall armyworm
SfMNPV isolates
Biological control
Baculovirus
Agricultural pest
description The fall armyworm, Spodoptera frugiperda (JE Smith) is a key pest in the Americas. Control strategies are mainly carried out by use of chemical insecticides and transgenic crops expressing Bacillus thuringiensis toxins. In the last years, resistance of S. frugiperda populations to transgenic corn was reported in different Latin American countries. The baculovirus Spodoptera frugiperda Multiple Nucleopolyhedrovirus (SfMNPV) is a pathogenic agent for the fall armyworm and a potential alternative for its control in integrated pest management strategies. In this work, we analyze some characteristics of two baculovirus isolates collected from maize (SfMNPV-M) and cotton (SfMNPV-C) fields from Argentina. The isolates were compared by restriction enzymes patterns and the analysis reveals the presence of genotypic variants in the SfMNPV-M isolate. We confirmed a deletion by sequencing fragments encompassing egt gene and most part of its contiguous gene (orf A) in a SfMNVP-M genotypic variant. Additionally, we estimated the 50% lethal dose and median survival time of each isolate in bioassays with S. frugiperda larvae.
format Articulo
Articulo
author Niz, José María
Salvador, Ricardo
Ferrelli, María Leticia
Sciocco de Cap, Alicia
Romanowski, Víctor
Berretta, Marcelo Facundo
author_facet Niz, José María
Salvador, Ricardo
Ferrelli, María Leticia
Sciocco de Cap, Alicia
Romanowski, Víctor
Berretta, Marcelo Facundo
author_sort Niz, José María
title Genetic variants in Argentinean isolates of Spodoptera frugiperda Multiple Nucleopolyhedrovirus
title_short Genetic variants in Argentinean isolates of Spodoptera frugiperda Multiple Nucleopolyhedrovirus
title_full Genetic variants in Argentinean isolates of Spodoptera frugiperda Multiple Nucleopolyhedrovirus
title_fullStr Genetic variants in Argentinean isolates of Spodoptera frugiperda Multiple Nucleopolyhedrovirus
title_full_unstemmed Genetic variants in Argentinean isolates of Spodoptera frugiperda Multiple Nucleopolyhedrovirus
title_sort genetic variants in argentinean isolates of spodoptera frugiperda multiple nucleopolyhedrovirus
publishDate 2020
url http://sedici.unlp.edu.ar/handle/10915/140243
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AT salvadorricardo geneticvariantsinargentineanisolatesofspodopterafrugiperdamultiplenucleopolyhedrovirus
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