Dynamics of maternal gene expression in Rhodnius prolixus

The study of the developmental processes in Rhodnius prolixus has recently advanced with the sequencing of the genome. In this work, we study maternal gene expression driving oogenesis and early embryogenesis in R. prolixus. We analyze the transcriptional profile of mRNAs to establish the genes expr...

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Detalles Bibliográficos
Autores principales: Pascual, Agustina, Rivera Pomar, Rolando Víctor
Formato: Articulo Preprint
Lenguaje:Inglés
Publicado: 2021
Materias:
Ciencias Exactas
Biología
oogenesis
early embryogenesis
gene expression
Hemiptera
hemimetabolous
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/140169
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-140169
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Biología
oogenesis
early embryogenesis
gene expression
Hemiptera
hemimetabolous
spellingShingle Ciencias Exactas
Biología
oogenesis
early embryogenesis
gene expression
Hemiptera
hemimetabolous
Pascual, Agustina
Rivera Pomar, Rolando Víctor
Dynamics of maternal gene expression in Rhodnius prolixus
topic_facet Ciencias Exactas
Biología
oogenesis
early embryogenesis
gene expression
Hemiptera
hemimetabolous
description The study of the developmental processes in Rhodnius prolixus has recently advanced with the sequencing of the genome. In this work, we study maternal gene expression driving oogenesis and early embryogenesis in R. prolixus. We analyze the transcriptional profile of mRNAs to establish the genes expressed across the ovary, unfertilized eggs and different embryonic stages of R. prolixus until the formation of the germ band anlage (0, 12, 24, and 48 hours post egg laying). We identified 81 putative maternal and ovary-related genes and validated their expression by qRT-PCR. Consistent with a role in oogenesis and early development of R. prolixus, we show that parental RNAi against Rp-BicD results in embryos that did not show any distinguishable embryonic structure. In this framework we propose three hierarchies of maternal genes that affect early and late oogenesis, and embryonic patterning.
format Articulo
Preprint
author Pascual, Agustina
Rivera Pomar, Rolando Víctor
author_facet Pascual, Agustina
Rivera Pomar, Rolando Víctor
author_sort Pascual, Agustina
title Dynamics of maternal gene expression in Rhodnius prolixus
title_short Dynamics of maternal gene expression in Rhodnius prolixus
title_full Dynamics of maternal gene expression in Rhodnius prolixus
title_fullStr Dynamics of maternal gene expression in Rhodnius prolixus
title_full_unstemmed Dynamics of maternal gene expression in Rhodnius prolixus
title_sort dynamics of maternal gene expression in rhodnius prolixus
publishDate 2021
url http://sedici.unlp.edu.ar/handle/10915/140169
work_keys_str_mv AT pascualagustina dynamicsofmaternalgeneexpressioninrhodniusprolixus
AT riverapomarrolandovictor dynamicsofmaternalgeneexpressioninrhodniusprolixus
bdutipo_str Repositorios
_version_ 1764820458339827713

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