Lipid Metabolism in Rats is Modified by Nitric Oxide Availability Through a Ca⁺⁺-Dependent Mechanism

We studied lipid metabolism and the antioxidant defense system in plasma and liver of rats fed diets supplemented with L<sup>ω</sup>-nitro-L-arginine methyl ester (L-NAME), isosorbide dinitrate (DIS), L-arginine (Arg), or the associations of these drugs. Liver hydroperoxide and thiobarbi...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Marra, Carlos Alberto, Nella, Julio, Manti, Damián, Tacconi de Alaniz, María Josefa
Formato: Articulo
Lenguaje:Inglés
Publicado: 2007
Materias:
Ciencias Médicas
Bioquímica
Oxidative stress
Calcium
Lipid metabolism
Rat liver
Nitric oxide
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/140165
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-140165
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Médicas
Bioquímica
Oxidative stress
Calcium
Lipid metabolism
Rat liver
Nitric oxide
spellingShingle Ciencias Médicas
Bioquímica
Oxidative stress
Calcium
Lipid metabolism
Rat liver
Nitric oxide
Marra, Carlos Alberto
Nella, Julio
Manti, Damián
Tacconi de Alaniz, María Josefa
Lipid Metabolism in Rats is Modified by Nitric Oxide Availability Through a Ca⁺⁺-Dependent Mechanism
topic_facet Ciencias Médicas
Bioquímica
Oxidative stress
Calcium
Lipid metabolism
Rat liver
Nitric oxide
description We studied lipid metabolism and the antioxidant defense system in plasma and liver of rats fed diets supplemented with L<sup>ω</sup>-nitro-L-arginine methyl ester (L-NAME), isosorbide dinitrate (DIS), L-arginine (Arg), or the associations of these drugs. Liver hydroperoxide and thiobarbituric-acid-reactive substance (TBARS) levels were decreased by Arg and increased by L-NAME or DIS treatments. Oxidized glutathione and conjugated dienes were increased by DIS. Nitrate + nitrite levels and serum calcium ([Ca⁺⁺]) were incremented by Arg or DIS and reduced by L-NAME. Superoxide dismutase and catalase activities decreased under Arg treatment, while L-NAME or DIS caused stimulation. Liver high-density lipoprotein (HDL) cholesterol was increased by DIS or NAME (alone or associated with Arg). Free fatty acids and neutral and polar lipids were increased by Arg, L-NAME, and DIS. However, predominating phospholipid synthesis increased the neutral/polar ratio. Decreased levels of nitric oxide (NO) (low [Ca⁺⁺]) was directly associated with increased fatty acid synthetase, decreased phospholipase A₂, carnitine-palmitoyl transferase, and fatty acid desaturase activities. Raised NO (high [Ca⁺⁺]) inversely correlated with increased phospholipase-A₂ and acyl-coenzyme A (CoA) synthetase and decreased fatty acid synthetase and β-oxidation rate. Arg or DIS produced changes that were partially reverted by association with L-NAME. Based on these observations, prolonged therapeutical approaches using drugs that modify NO availability should be carefully considered.
format Articulo
Articulo
author Marra, Carlos Alberto
Nella, Julio
Manti, Damián
Tacconi de Alaniz, María Josefa
author_facet Marra, Carlos Alberto
Nella, Julio
Manti, Damián
Tacconi de Alaniz, María Josefa
author_sort Marra, Carlos Alberto
title Lipid Metabolism in Rats is Modified by Nitric Oxide Availability Through a Ca⁺⁺-Dependent Mechanism
title_short Lipid Metabolism in Rats is Modified by Nitric Oxide Availability Through a Ca⁺⁺-Dependent Mechanism
title_full Lipid Metabolism in Rats is Modified by Nitric Oxide Availability Through a Ca⁺⁺-Dependent Mechanism
title_fullStr Lipid Metabolism in Rats is Modified by Nitric Oxide Availability Through a Ca⁺⁺-Dependent Mechanism
title_full_unstemmed Lipid Metabolism in Rats is Modified by Nitric Oxide Availability Through a Ca⁺⁺-Dependent Mechanism
title_sort lipid metabolism in rats is modified by nitric oxide availability through a ca⁺⁺-dependent mechanism
publishDate 2007
url http://sedici.unlp.edu.ar/handle/10915/140165
work_keys_str_mv AT marracarlosalberto lipidmetabolisminratsismodifiedbynitricoxideavailabilitythroughacadependentmechanism
AT nellajulio lipidmetabolisminratsismodifiedbynitricoxideavailabilitythroughacadependentmechanism
AT mantidamian lipidmetabolisminratsismodifiedbynitricoxideavailabilitythroughacadependentmechanism
AT tacconidealanizmariajosefa lipidmetabolisminratsismodifiedbynitricoxideavailabilitythroughacadependentmechanism
bdutipo_str Repositorios
_version_ 1764820458337730560

Ejemplares similares