Fatty acid-binding proteins in Echinococcus spp.: the family has grown

Fatty acid-binding proteins (FABPs) are small intracellular proteins that reversibly bind fatty acids and other hydrophobic ligands. In cestodes, due to their inability to synthesise fatty acids de novo, FABPs have been proposed as essential proteins, and thus, as possible drug targets and/or carrie...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Pórfido, Jorge Luis, Herz, Michaela, Kiss, Ferenc, Kamenetzky, Laura, Brehm, Klaus, Rosenzvit, Mara Cecilia, Córsico, Betina, Franchini, Gisela Raquel
Formato: Articulo
Lenguaje:Inglés
Publicado: 2020
Materias:
Bioquímica
Ciencias Exactas
Echinococcus multilocularis
FABPs
Fatty acids
Cestodes
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/140067
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-140067
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Bioquímica
Ciencias Exactas
Echinococcus multilocularis
FABPs
Fatty acids
Cestodes
spellingShingle Bioquímica
Ciencias Exactas
Echinococcus multilocularis
FABPs
Fatty acids
Cestodes
Pórfido, Jorge Luis
Herz, Michaela
Kiss, Ferenc
Kamenetzky, Laura
Brehm, Klaus
Rosenzvit, Mara Cecilia
Córsico, Betina
Franchini, Gisela Raquel
Fatty acid-binding proteins in Echinococcus spp.: the family has grown
topic_facet Bioquímica
Ciencias Exactas
Echinococcus multilocularis
FABPs
Fatty acids
Cestodes
description Fatty acid-binding proteins (FABPs) are small intracellular proteins that reversibly bind fatty acids and other hydrophobic ligands. In cestodes, due to their inability to synthesise fatty acids de novo, FABPs have been proposed as essential proteins, and thus, as possible drug targets and/or carriers against these parasites. We performed data mining in Echinococcus multilocularis and Echinococcus granulosus genomes in order to test whether this family of proteins is more complex than previously reported. By exploring the genomes of E. multilocularis and E. granulosus, six genes coding for FABPs were found in each organism. In the case of E. granulosus, all of them have different coding sequences, whereas in E. multilocularis, two of the genes code for the same protein. Remarkably, one of the genes (in both cestodes) encodes a FABP with a C-terminal extension unusual for this family of proteins. The newly described genes present variations in their structure in comparison with previously described FABP genes in Echinococcus spp. The coding sequences for E. multilocularis were validated by cloning and sequencing. Moreover, differential expression patterns of FABPs were observed at different stages of the life cycle of E. multilocularis by exploring transcriptomic data from several sources. In summary, FABP family in cestodes is far more complex than previously thought and includes new members that seem to be only present in flatworms.
format Articulo
Articulo
author Pórfido, Jorge Luis
Herz, Michaela
Kiss, Ferenc
Kamenetzky, Laura
Brehm, Klaus
Rosenzvit, Mara Cecilia
Córsico, Betina
Franchini, Gisela Raquel
author_facet Pórfido, Jorge Luis
Herz, Michaela
Kiss, Ferenc
Kamenetzky, Laura
Brehm, Klaus
Rosenzvit, Mara Cecilia
Córsico, Betina
Franchini, Gisela Raquel
author_sort Pórfido, Jorge Luis
title Fatty acid-binding proteins in Echinococcus spp.: the family has grown
title_short Fatty acid-binding proteins in Echinococcus spp.: the family has grown
title_full Fatty acid-binding proteins in Echinococcus spp.: the family has grown
title_fullStr Fatty acid-binding proteins in Echinococcus spp.: the family has grown
title_full_unstemmed Fatty acid-binding proteins in Echinococcus spp.: the family has grown
title_sort fatty acid-binding proteins in echinococcus spp.: the family has grown
publishDate 2020
url http://sedici.unlp.edu.ar/handle/10915/140067
work_keys_str_mv AT porfidojorgeluis fattyacidbindingproteinsinechinococcussppthefamilyhasgrown
AT herzmichaela fattyacidbindingproteinsinechinococcussppthefamilyhasgrown
AT kissferenc fattyacidbindingproteinsinechinococcussppthefamilyhasgrown
AT kamenetzkylaura fattyacidbindingproteinsinechinococcussppthefamilyhasgrown
AT brehmklaus fattyacidbindingproteinsinechinococcussppthefamilyhasgrown
AT rosenzvitmaracecilia fattyacidbindingproteinsinechinococcussppthefamilyhasgrown
AT corsicobetina fattyacidbindingproteinsinechinococcussppthefamilyhasgrown
AT franchinigiselaraquel fattyacidbindingproteinsinechinococcussppthefamilyhasgrown
bdutipo_str Repositorios
_version_ 1764820458233921537

Ejemplares similares