Amitraz induced cytotoxic effect on bovine cumulus cells and impaired oocyte maturation

The aim of this study was to evaluate the genotoxic and cytotoxic effects of amitraz (AMZ) on the primary culture of bovine cumulus cells (CC) and oocyte nuclear maturation. Cytotoxicity was evaluated by assessing mitochondrial activity with the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazoli...

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Autores principales: Nikoloff, Noelia, Carranza Martín, Ana Cristina, Fabra, Mariana Carolina, Furnus, Cecilia Cristina
Formato: Articulo
Lenguaje:Inglés
Publicado: 2021
Materias:
Veterinaria
Formamide pesticide
Cumulus-oocyte complex
Apoptosis
MTT
Single-cell gel electrophoresis
Oocyte nuclear maturation
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/139973
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-139973
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Veterinaria
Formamide pesticide
Cumulus-oocyte complex
Apoptosis
MTT
Single-cell gel electrophoresis
Oocyte nuclear maturation
spellingShingle Veterinaria
Formamide pesticide
Cumulus-oocyte complex
Apoptosis
MTT
Single-cell gel electrophoresis
Oocyte nuclear maturation
Nikoloff, Noelia
Carranza Martín, Ana Cristina
Fabra, Mariana Carolina
Furnus, Cecilia Cristina
Amitraz induced cytotoxic effect on bovine cumulus cells and impaired oocyte maturation
topic_facet Veterinaria
Formamide pesticide
Cumulus-oocyte complex
Apoptosis
MTT
Single-cell gel electrophoresis
Oocyte nuclear maturation
description The aim of this study was to evaluate the genotoxic and cytotoxic effects of amitraz (AMZ) on the primary culture of bovine cumulus cells (CC) and oocyte nuclear maturation. Cytotoxicity was evaluated by assessing mitochondrial activity with the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. Genotoxicity was estimated using the alkaline single cell gel electrophoresis (SCGE) assay. Apoptosis was detected with the Annexin V-affinity assay. The in vitro maturation test was performed in bovine oocytes. To understand AMZ action, glutathione content, superoxide dismutase enzyme activity, and lipid peroxidation were evaluated in CC. Results showed that AMZ lethal concentration (LC 50<sub>24h</sub>) for bovine CC was 32.55 μg/mL (MTT assay). A 25 μg/mL induced late apoptosis and necrotic cells (p < 0.05); however, DNA damage was decreased at the same concentration (SCGE assay; p < 0.05). A decrease in metaphase II was observed at 25 μg/mL, and degenerate oocytes were observed at 15 and 25 μg/mL (p < 0.05). None of the oxidative stress parameters evaluated showed significant differences. This study contributes to a better understanding of AMZ in this model, suggesting its potential cytotoxicity and impact on bovine reproduction.
format Articulo
Articulo
author Nikoloff, Noelia
Carranza Martín, Ana Cristina
Fabra, Mariana Carolina
Furnus, Cecilia Cristina
author_facet Nikoloff, Noelia
Carranza Martín, Ana Cristina
Fabra, Mariana Carolina
Furnus, Cecilia Cristina
author_sort Nikoloff, Noelia
title Amitraz induced cytotoxic effect on bovine cumulus cells and impaired oocyte maturation
title_short Amitraz induced cytotoxic effect on bovine cumulus cells and impaired oocyte maturation
title_full Amitraz induced cytotoxic effect on bovine cumulus cells and impaired oocyte maturation
title_fullStr Amitraz induced cytotoxic effect on bovine cumulus cells and impaired oocyte maturation
title_full_unstemmed Amitraz induced cytotoxic effect on bovine cumulus cells and impaired oocyte maturation
title_sort amitraz induced cytotoxic effect on bovine cumulus cells and impaired oocyte maturation
publishDate 2021
url http://sedici.unlp.edu.ar/handle/10915/139973
work_keys_str_mv AT nikoloffnoelia amitrazinducedcytotoxiceffectonbovinecumuluscellsandimpairedoocytematuration
AT carranzamartinanacristina amitrazinducedcytotoxiceffectonbovinecumuluscellsandimpairedoocytematuration
AT fabramarianacarolina amitrazinducedcytotoxiceffectonbovinecumuluscellsandimpairedoocytematuration
AT furnusceciliacristina amitrazinducedcytotoxiceffectonbovinecumuluscellsandimpairedoocytematuration
bdutipo_str Repositorios
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