Effects of Erythrocytes Treated with Alpha Hemolysin of E.Coli on Endothelial Cells

Uropathogenic strains of E. coli deliver the toxin alpha-hemolysin (HlyA) to optimize the host environment for the spread of infection. It was reported that at high concentrations, the toxin forms pores in eukaryotic membranes, leading to cell lysis, while lower concentrations might interfere with h...

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Detalles Bibliográficos
Autores principales: Herlax, Vanesa Silvana, Denis, María Florencia Leal, Alvarez, Cora Lilia, Lefevre, Sophie D., Enrique, Nicolás Jorge, Maté, Sabina María, Milesi, María Verónica, Ostuni, Mariano A., Schwarzbaum, Pablo J.
Formato: Articulo Contribucion a revista
Lenguaje:Inglés
Publicado: 2017
Materias:
Medicina
Biología
E. coli
infection
osmoscan experiments
erythrocytes
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/139712
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-139712
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Medicina
Biología
E. coli
infection
osmoscan experiments
erythrocytes
spellingShingle Medicina
Biología
E. coli
infection
osmoscan experiments
erythrocytes
Herlax, Vanesa Silvana
Denis, María Florencia Leal
Alvarez, Cora Lilia
Lefevre, Sophie D.
Enrique, Nicolás Jorge
Maté, Sabina María
Milesi, María Verónica
Ostuni, Mariano A.
Schwarzbaum, Pablo J.
Effects of Erythrocytes Treated with Alpha Hemolysin of E.Coli on Endothelial Cells
topic_facet Medicina
Biología
E. coli
infection
osmoscan experiments
erythrocytes
description Uropathogenic strains of E. coli deliver the toxin alpha-hemolysin (HlyA) to optimize the host environment for the spread of infection. It was reported that at high concentrations, the toxin forms pores in eukaryotic membranes, leading to cell lysis, while lower concentrations might interfere with host-cell-signaling pathways, causing apoptosis. In the present investigation we demonstrate that a relatively low concentration of HlyA induces morphological changes and phosphatidylserine (PS) externalization of human erythrocytes. On the other hand, the unacylated nonhemolytic form of HlyA, ProHlyA induces similar morphological changes but no PS externalization. We performed osmoscan experiments to test the effect of both proteins on erythrocytes structure.
format Articulo
Contribucion a revista
author Herlax, Vanesa Silvana
Denis, María Florencia Leal
Alvarez, Cora Lilia
Lefevre, Sophie D.
Enrique, Nicolás Jorge
Maté, Sabina María
Milesi, María Verónica
Ostuni, Mariano A.
Schwarzbaum, Pablo J.
author_facet Herlax, Vanesa Silvana
Denis, María Florencia Leal
Alvarez, Cora Lilia
Lefevre, Sophie D.
Enrique, Nicolás Jorge
Maté, Sabina María
Milesi, María Verónica
Ostuni, Mariano A.
Schwarzbaum, Pablo J.
author_sort Herlax, Vanesa Silvana
title Effects of Erythrocytes Treated with Alpha Hemolysin of E.Coli on Endothelial Cells
title_short Effects of Erythrocytes Treated with Alpha Hemolysin of E.Coli on Endothelial Cells
title_full Effects of Erythrocytes Treated with Alpha Hemolysin of E.Coli on Endothelial Cells
title_fullStr Effects of Erythrocytes Treated with Alpha Hemolysin of E.Coli on Endothelial Cells
title_full_unstemmed Effects of Erythrocytes Treated with Alpha Hemolysin of E.Coli on Endothelial Cells
title_sort effects of erythrocytes treated with alpha hemolysin of e.coli on endothelial cells
publishDate 2017
url http://sedici.unlp.edu.ar/handle/10915/139712
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