On the Thermal Stability of DNA in Solution of Mixed Solvents

The study of the changes in UV absorbance of DNA solutions in water/dioxane and water/ethylene glycol mixture at different concentrations shows that the thermal denaturation of DNA is sensitive to the electrical permittivity of the media and the water content. At relative low concentrations of co-so...

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Detalles Bibliográficos
Autores principales: Xammar Oro, Juan Roberto de, Grigera, José Raúl
Formato: Articulo
Lenguaje:Inglés
Publicado: 1995
Materias:
Física
DNA stability
hydrophobic interactions
water stabilization effects
electrostatic interactions in DNA
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/139453
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-139453
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Física
DNA stability
hydrophobic interactions
water stabilization effects
electrostatic interactions in DNA
spellingShingle Física
DNA stability
hydrophobic interactions
water stabilization effects
electrostatic interactions in DNA
Xammar Oro, Juan Roberto de
Grigera, José Raúl
On the Thermal Stability of DNA in Solution of Mixed Solvents
topic_facet Física
DNA stability
hydrophobic interactions
water stabilization effects
electrostatic interactions in DNA
description The study of the changes in UV absorbance of DNA solutions in water/dioxane and water/ethylene glycol mixture at different concentrations shows that the thermal denaturation of DNA is sensitive to the electrical permittivity of the media and the water content. At relative low concentrations of co-solvent the dominant feature is the electrical permittivity. When water content is lower than a critical value, the electrical permittivity is no longer the determinant of the denaturation temperature but the partial volume fraction of water. The critical water content is about 0.69 partial volume fraction of water.
format Articulo
Articulo
author Xammar Oro, Juan Roberto de
Grigera, José Raúl
author_facet Xammar Oro, Juan Roberto de
Grigera, José Raúl
author_sort Xammar Oro, Juan Roberto de
title On the Thermal Stability of DNA in Solution of Mixed Solvents
title_short On the Thermal Stability of DNA in Solution of Mixed Solvents
title_full On the Thermal Stability of DNA in Solution of Mixed Solvents
title_fullStr On the Thermal Stability of DNA in Solution of Mixed Solvents
title_full_unstemmed On the Thermal Stability of DNA in Solution of Mixed Solvents
title_sort on the thermal stability of dna in solution of mixed solvents
publishDate 1995
url http://sedici.unlp.edu.ar/handle/10915/139453
work_keys_str_mv AT xammarorojuanrobertode onthethermalstabilityofdnainsolutionofmixedsolvents
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bdutipo_str Repositorios
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