Inflammatory changes after cryosurgery-induced necrosis in human melanoma xenografted in nude mice

There is growing evidence that necrosis, instead of apoptosis, could act as a natural adjuvant, which could activate an immune response. In this work we have investigated if induction of tumor necrosis could trigger the affluence of inflammatory cells at the tumor site, and thus induce an immune res...

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Autores principales:	Gazzaniga, Silvina, Bravo, Alicia I., Goldszmid, Silvana R., Maschi, Fabricio Alejandro, Martinelli, Julio, Mordoh, José, Wainstok, Rosa
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2001
Materias:	Veterinaria Medicina cryosurgery granulocyte-monocyte colony-stimulating factor inflammatory infiltrate melanoma
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/139317
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-139317
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Veterinaria Medicina cryosurgery granulocyte-monocyte colony-stimulating factor inflammatory infiltrate melanoma
spellingShingle	Veterinaria Medicina cryosurgery granulocyte-monocyte colony-stimulating factor inflammatory infiltrate melanoma Gazzaniga, Silvina Bravo, Alicia I. Goldszmid, Silvana R. Maschi, Fabricio Alejandro Martinelli, Julio Mordoh, José Wainstok, Rosa Inflammatory changes after cryosurgery-induced necrosis in human melanoma xenografted in nude mice
topic_facet	Veterinaria Medicina cryosurgery granulocyte-monocyte colony-stimulating factor inflammatory infiltrate melanoma
description	There is growing evidence that necrosis, instead of apoptosis, could act as a natural adjuvant, which could activate an immune response. In this work we have investigated if induction of tumor necrosis could trigger the affluence of inflammatory cells at the tumor site, and thus induce an immune response. For this purpose, a liquid N 2 spray was applied on human melanoma (IIB-MEL-J cell line) xenografted in nude mice and 24 h later some mice received intratumorally a single 500 U dose of recombinant murine granulocyte macrophage-colony-stimulating factor. 77–100% of the tumor mass underwent necrosis. Congestion, edema, and endothelial cell activation were the first noticeable events. A quick infiltrative response of polymorphonuclear leukocytes around the tumor was detected 24 h after liquid N 2 application, peaking at day 3. Massive macrophage recruitment was observed since day 3. An early intratumoral infiltration with inflammatory cells was only detected in the group that received recombinant murine granulocyte macrophage- colony-stimulating factor after necrosis induction by liquid N 2. Coexisting DEC 205- and F4/80-positive cells increased in number, and their localization was predominantly peritumoral after necrosis. Antibody response was only detected in the groups with tumor-induced necrosis. Our results suggest that cryosurgery-induced necrosis could be a useful model to analyze the interaction among necrosis, inflammation, and the generation of an immune response.
format	Articulo Articulo
author	Gazzaniga, Silvina Bravo, Alicia I. Goldszmid, Silvana R. Maschi, Fabricio Alejandro Martinelli, Julio Mordoh, José Wainstok, Rosa
author_facet	Gazzaniga, Silvina Bravo, Alicia I. Goldszmid, Silvana R. Maschi, Fabricio Alejandro Martinelli, Julio Mordoh, José Wainstok, Rosa
author_sort	Gazzaniga, Silvina
title	Inflammatory changes after cryosurgery-induced necrosis in human melanoma xenografted in nude mice
title_short	Inflammatory changes after cryosurgery-induced necrosis in human melanoma xenografted in nude mice
title_full	Inflammatory changes after cryosurgery-induced necrosis in human melanoma xenografted in nude mice
title_fullStr	Inflammatory changes after cryosurgery-induced necrosis in human melanoma xenografted in nude mice
title_full_unstemmed	Inflammatory changes after cryosurgery-induced necrosis in human melanoma xenografted in nude mice
title_sort	inflammatory changes after cryosurgery-induced necrosis in human melanoma xenografted in nude mice
publishDate	2001
url	http://sedici.unlp.edu.ar/handle/10915/139317
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bdutipo_str	Repositorios
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Inflammatory changes after cryosurgery-induced necrosis in human melanoma xenografted in nude mice

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