Enzyme-Based Most Probable Number Method for the Enumeration of <i>Bifidobacterium</i> in Dairy Products

An enzyme-based assay in combination with the most probable number (MPN) technique was developed for the enumeration of bifidobacteria. The assay employs the detection of fructose-6-phosphate phosphoketolase (F6PPK) activity as an indicator of the presence of bifidobacteria. The method was validated...

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Detalles Bibliográficos
Autores principales: Bibiloni, Rodrigo, Gómez-Zavaglia, Andrea, De Antoni, Graciela Liliana
Formato: Articulo
Lenguaje:Inglés
Publicado: 2001
Materias:
Ciencias Exactas
Biología
Bifidobacterium
Enzyme
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/139269
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-139269
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Biología
Bifidobacterium
Enzyme
spellingShingle Ciencias Exactas
Biología
Bifidobacterium
Enzyme
Bibiloni, Rodrigo
Gómez-Zavaglia, Andrea
De Antoni, Graciela Liliana
Enzyme-Based Most Probable Number Method for the Enumeration of <i>Bifidobacterium</i> in Dairy Products
topic_facet Ciencias Exactas
Biología
Bifidobacterium
Enzyme
description An enzyme-based assay in combination with the most probable number (MPN) technique was developed for the enumeration of bifidobacteria. The assay employs the detection of fructose-6-phosphate phosphoketolase (F6PPK) activity as an indicator of the presence of bifidobacteria. The method was validated against viable counts and optimized with respect to selective media in order to quantitatively assess bifidobacteria in dairy products and other probiotic preparations. Several commercial products and homemade fermented milks were analyzed. Counts of bifidobacteria ranged from 10⁷ to 10⁸ MPN/ml in commercial products and homemade fermented milks Commercial starters provided by Argentinean industries had between 10⁷ and 10¹¹ MPN/ml. The results obtained in this study suggest that the combination of F6PPK activity determination and the MPN methodology allows an accurate determination of <i>Bifidobacterium</i> in pure cultures, dairy products, and other probiotic preparations.
format Articulo
Articulo
author Bibiloni, Rodrigo
Gómez-Zavaglia, Andrea
De Antoni, Graciela Liliana
author_facet Bibiloni, Rodrigo
Gómez-Zavaglia, Andrea
De Antoni, Graciela Liliana
author_sort Bibiloni, Rodrigo
title Enzyme-Based Most Probable Number Method for the Enumeration of <i>Bifidobacterium</i> in Dairy Products
title_short Enzyme-Based Most Probable Number Method for the Enumeration of <i>Bifidobacterium</i> in Dairy Products
title_full Enzyme-Based Most Probable Number Method for the Enumeration of <i>Bifidobacterium</i> in Dairy Products
title_fullStr Enzyme-Based Most Probable Number Method for the Enumeration of <i>Bifidobacterium</i> in Dairy Products
title_full_unstemmed Enzyme-Based Most Probable Number Method for the Enumeration of <i>Bifidobacterium</i> in Dairy Products
title_sort enzyme-based most probable number method for the enumeration of <i>bifidobacterium</i> in dairy products
publishDate 2001
url http://sedici.unlp.edu.ar/handle/10915/139269
work_keys_str_mv AT bibilonirodrigo enzymebasedmostprobablenumbermethodfortheenumerationofibifidobacteriumiindairyproducts
AT gomezzavagliaandrea enzymebasedmostprobablenumbermethodfortheenumerationofibifidobacteriumiindairyproducts
AT deantonigracielaliliana enzymebasedmostprobablenumbermethodfortheenumerationofibifidobacteriumiindairyproducts
bdutipo_str Repositorios
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