Effect of "fasting" and different concentrations of glucose on alpha-linolenic acid metabolism in HTC cells: correlation with the ultrastructural study
HTC cells are able to convert α-linolenic acid into higher homologs by desaturating and elongating reactions. When the cells were cultured in a Krebs Ringer bicarbonate solution (“fasted cells”) a decrease in both biosynthetic reactions took place. “Refeeding” the cells with Swim's 77 medium wi...
| Autores principales: | , |
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| Formato: | Articulo |
| Lenguaje: | Inglés |
| Publicado: |
1977
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| Materias: | |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/139082 |
| Aporte de: |
| id |
I19-R120-10915-139082 |
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| record_format |
dspace |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Bioquímica Glucose Bicarbonate Polyunsaturated fatty acid Acid Metabolism Complete medium |
| spellingShingle |
Bioquímica Glucose Bicarbonate Polyunsaturated fatty acid Acid Metabolism Complete medium Gómez Dumm, César Leandro Alberto Tacconi de Alaniz, María Josefa Effect of "fasting" and different concentrations of glucose on alpha-linolenic acid metabolism in HTC cells: correlation with the ultrastructural study |
| topic_facet |
Bioquímica Glucose Bicarbonate Polyunsaturated fatty acid Acid Metabolism Complete medium |
| description |
HTC cells are able to convert α-linolenic acid into higher homologs by desaturating and elongating reactions. When the cells were cultured in a Krebs Ringer bicarbonate solution (“fasted cells”) a decrease in both biosynthetic reactions took place. “Refeeding” the cells with Swim's 77 medium without glucose enhanced the biosynthesis of polyunsaturated fatty acids from α-linolenic acid family, but when glucose was added to the medium, α-linolenic acid was preferably elongated rather than converted into eicose-pentaenoic acid.
The ultrastructural study revealed HTC cells with a simple cytoplasmic organization, showing little evidence of their origin from hepatocytes. The cells cultured in a complete medium appeared well preserved and were similar to those “fasted” for 12 hours and “refed” for another 12 hours using Swim's 77 medium without serum. The amount of glucose in the medium plays a role in preserving the cell structure. This effect does not occur if glucose is added in the absence of aminoacids and vitamins. |
| format |
Articulo Articulo |
| author |
Gómez Dumm, César Leandro Alberto Tacconi de Alaniz, María Josefa |
| author_facet |
Gómez Dumm, César Leandro Alberto Tacconi de Alaniz, María Josefa |
| author_sort |
Gómez Dumm, César Leandro Alberto |
| title |
Effect of "fasting" and different concentrations of glucose on alpha-linolenic acid metabolism in HTC cells: correlation with the ultrastructural study |
| title_short |
Effect of "fasting" and different concentrations of glucose on alpha-linolenic acid metabolism in HTC cells: correlation with the ultrastructural study |
| title_full |
Effect of "fasting" and different concentrations of glucose on alpha-linolenic acid metabolism in HTC cells: correlation with the ultrastructural study |
| title_fullStr |
Effect of "fasting" and different concentrations of glucose on alpha-linolenic acid metabolism in HTC cells: correlation with the ultrastructural study |
| title_full_unstemmed |
Effect of "fasting" and different concentrations of glucose on alpha-linolenic acid metabolism in HTC cells: correlation with the ultrastructural study |
| title_sort |
effect of "fasting" and different concentrations of glucose on alpha-linolenic acid metabolism in htc cells: correlation with the ultrastructural study |
| publishDate |
1977 |
| url |
http://sedici.unlp.edu.ar/handle/10915/139082 |
| work_keys_str_mv |
AT gomezdummcesarleandroalberto effectoffastinganddifferentconcentrationsofglucoseonalphalinolenicacidmetabolisminhtccellscorrelationwiththeultrastructuralstudy AT tacconidealanizmariajosefa effectoffastinganddifferentconcentrationsofglucoseonalphalinolenicacidmetabolisminhtccellscorrelationwiththeultrastructuralstudy |
| bdutipo_str |
Repositorios |
| _version_ |
1764820457012330499 |