The cloning of the virus envelope glycoprotein F of canine distemper virus expressed in Pichia pastoris

Canine distemper virus (CDV) is a pathogen which affects members of the Canidae family, causing an acute, often fatal, systemic disease. CDV is an RNA virus of the family Paramyxoviridae that contains two envelope glycoproteins: F and HA. In this study, we focused on the envelope glycoprotein F as t...

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Detalles Bibliográficos
Autores principales: Tizzano, Marco Antonio, Sguazza, Guillermo Hernán, Picotto, Leandro Daniel, Echeverría, María Gabriela, Pecoraro, Marcelo Ricardo Ítalo
Formato: Articulo
Lenguaje:Inglés
Publicado: 2020
Materias:
Veterinaria
Canine distemper virus
Glycoprotein F
Pichia pastoris
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/139073
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-139073
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Veterinaria
Canine distemper virus
Glycoprotein F
Pichia pastoris
spellingShingle Veterinaria
Canine distemper virus
Glycoprotein F
Pichia pastoris
Tizzano, Marco Antonio
Sguazza, Guillermo Hernán
Picotto, Leandro Daniel
Echeverría, María Gabriela
Pecoraro, Marcelo Ricardo Ítalo
The cloning of the virus envelope glycoprotein F of canine distemper virus expressed in Pichia pastoris
topic_facet Veterinaria
Canine distemper virus
Glycoprotein F
Pichia pastoris
description Canine distemper virus (CDV) is a pathogen which affects members of the Canidae family, causing an acute, often fatal, systemic disease. CDV is an RNA virus of the family Paramyxoviridae that contains two envelope glycoproteins: F and HA. In this study, we focused on the envelope glycoprotein F as the main target for neutralizing antibodies produced after infection or vaccination. The complete coding region of the protein (60 kDa) was expressed in the methylotrophic yeast Pichia pastoris, obtained in a recombinant form and secreted to the culture medium. Later, to analyze its immunogenicity, the protein was combined with an oily adjuvant and used to inoculate mice. The results provide evidence supporting a potential application of this recombinant protein as a subunit vaccine.
format Articulo
Articulo
author Tizzano, Marco Antonio
Sguazza, Guillermo Hernán
Picotto, Leandro Daniel
Echeverría, María Gabriela
Pecoraro, Marcelo Ricardo Ítalo
author_facet Tizzano, Marco Antonio
Sguazza, Guillermo Hernán
Picotto, Leandro Daniel
Echeverría, María Gabriela
Pecoraro, Marcelo Ricardo Ítalo
author_sort Tizzano, Marco Antonio
title The cloning of the virus envelope glycoprotein F of canine distemper virus expressed in Pichia pastoris
title_short The cloning of the virus envelope glycoprotein F of canine distemper virus expressed in Pichia pastoris
title_full The cloning of the virus envelope glycoprotein F of canine distemper virus expressed in Pichia pastoris
title_fullStr The cloning of the virus envelope glycoprotein F of canine distemper virus expressed in Pichia pastoris
title_full_unstemmed The cloning of the virus envelope glycoprotein F of canine distemper virus expressed in Pichia pastoris
title_sort cloning of the virus envelope glycoprotein f of canine distemper virus expressed in pichia pastoris
publishDate 2020
url http://sedici.unlp.edu.ar/handle/10915/139073
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