Cell interactions between a nonpathogenic <i>Fusarium oxysporum</i> strain and root tissues of <i>Eucalyptus viminalis</i>

Nonpathogenic isolates of <i>Fusarium oxysporum</i> can be successful antagonists of pathogenic forms of the same fungal species that commonly attacks crop plants. The characteristics that distinguish nonpathogenic from pathogenic forms are not well understood. In this study, the mode of...

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Autores principales: Salerno, María Isabel, Gianinazzi, Silvio, Arnould, Christine, Gianinazzi-Pearson, Vivienne
Formato: Articulo
Lenguaje:Inglés
Publicado: 2004
Materias:
Ciencias Agrarias
Nonpathogenic
Fusarium oxysporum
Eucalyptus viminalis
Cell interactions
Pectin
Ultrastructure
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/138960
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-138960
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Agrarias
Nonpathogenic
Fusarium oxysporum
Eucalyptus viminalis
Cell interactions
Pectin
Ultrastructure
spellingShingle Ciencias Agrarias
Nonpathogenic
Fusarium oxysporum
Eucalyptus viminalis
Cell interactions
Pectin
Ultrastructure
Salerno, María Isabel
Gianinazzi, Silvio
Arnould, Christine
Gianinazzi-Pearson, Vivienne
Cell interactions between a nonpathogenic <i>Fusarium oxysporum</i> strain and root tissues of <i>Eucalyptus viminalis</i>
topic_facet Ciencias Agrarias
Nonpathogenic
Fusarium oxysporum
Eucalyptus viminalis
Cell interactions
Pectin
Ultrastructure
description Nonpathogenic isolates of <i>Fusarium oxysporum</i> can be successful antagonists of pathogenic forms of the same fungal species that commonly attacks crop plants. The characteristics that distinguish nonpathogenic from pathogenic forms are not well understood. In this study, the mode of root colonization of <i>Eucalyptus viminalis</i> seedlings by a nonpathogenic <i>F. oxysporum</i> strain is described at the ultrastructural level. Root systems of <i>E. viminalis</i> plants were inoculated with nonpathogenic <i>F. oxysporum</i> strain Fo47 in an in vitro model system. Changes in the occurrence of nonesterified and methyl-esterified pectins in colonized <i>E. viminalis</i> roots were evaluated by in situ immunolabeling using two monoclonal antibodies, JIM 5 and JIM 7. Modes of penetration and root colonization patterns in <i>E. viminalis</i> seedlings by the nonpathogenic fungus were similar to those described for pathogenic forms of <i>F. oxysporum</i>. However, root interactions differed in that the nonpathogenic fungus did not induce host tissue damage. No papilla-like appositions were observed in host cells in response to invading hyphae, which did not disrupt the host plasma membrane in many cases, suggesting that a biotrophic relationship was established. Root colonization by the nonpathogenic strain did not induce alteration in JIM 7 labeling of methyl-esterified pectin in <i>E. viminalis</i> cell walls, whereas nonesterified pectin was detected to a significantly greater extent in cell walls of roots colonized by the fungus. Pectin components decreased slightly only at points of hyphal contact with host cells. Because nonpathogenic strains utilize pectin in pure culture, host control over enzyme activity or production by the fungi may at least partly explain their compatible interactions with host tissues.
format Articulo
Articulo
author Salerno, María Isabel
Gianinazzi, Silvio
Arnould, Christine
Gianinazzi-Pearson, Vivienne
author_facet Salerno, María Isabel
Gianinazzi, Silvio
Arnould, Christine
Gianinazzi-Pearson, Vivienne
author_sort Salerno, María Isabel
title Cell interactions between a nonpathogenic <i>Fusarium oxysporum</i> strain and root tissues of <i>Eucalyptus viminalis</i>
title_short Cell interactions between a nonpathogenic <i>Fusarium oxysporum</i> strain and root tissues of <i>Eucalyptus viminalis</i>
title_full Cell interactions between a nonpathogenic <i>Fusarium oxysporum</i> strain and root tissues of <i>Eucalyptus viminalis</i>
title_fullStr Cell interactions between a nonpathogenic <i>Fusarium oxysporum</i> strain and root tissues of <i>Eucalyptus viminalis</i>
title_full_unstemmed Cell interactions between a nonpathogenic <i>Fusarium oxysporum</i> strain and root tissues of <i>Eucalyptus viminalis</i>
title_sort cell interactions between a nonpathogenic <i>fusarium oxysporum</i> strain and root tissues of <i>eucalyptus viminalis</i>
publishDate 2004
url http://sedici.unlp.edu.ar/handle/10915/138960
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