Growth and protopectinase production of <i>Geotrichum klebahnii</i> in batch and continuous cultures with synthetic media

ATCC 42397 produces a protopectinase (PPase-SE) with polygalacturonase (PGase) activity. The microorganism was aerobically cultivated in synthetic media. Glucose, fructose and xylose yielded the highest enzyme levels (10–11 PGase units ml−1). Galacturonic acid repressed enzyme production and no grow...

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Detalles Bibliográficos
Autores principales: Cavalitto, Sebastián Fernando, Hours, Roque Alberto, Mignone, Carlos Fernando
Formato: Articulo
Lenguaje:Inglés
Publicado: 2000
Materias:
Ciencias Exactas
Pectin
Pectin solubilizing enzimes
Protopectinases
Polygalacturonases
Geotrichum
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/138739
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-138739
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Pectin
Pectin solubilizing enzimes
Protopectinases
Polygalacturonases
Geotrichum
spellingShingle Ciencias Exactas
Pectin
Pectin solubilizing enzimes
Protopectinases
Polygalacturonases
Geotrichum
Cavalitto, Sebastián Fernando
Hours, Roque Alberto
Mignone, Carlos Fernando
Growth and protopectinase production of <i>Geotrichum klebahnii</i> in batch and continuous cultures with synthetic media
topic_facet Ciencias Exactas
Pectin
Pectin solubilizing enzimes
Protopectinases
Polygalacturonases
Geotrichum
description ATCC 42397 produces a protopectinase (PPase-SE) with polygalacturonase (PGase) activity. The microorganism was aerobically cultivated in synthetic media. Glucose, fructose and xylose yielded the highest enzyme levels (10–11 PGase units ml−1). Galacturonic acid repressed enzyme production and no growth was obtained with disaccharides and pectin. Specific enzyme activity obtained in an O2-limited culture was similar to that found in nonlimited ones. A growth yield (Y; x/s) of 0.49 g of cell dry weight per gram of glucose consumed was obtained in a typical batch bioreactor culture. Enzyme production was growth associated, and no major products other than biomass and CO2 were detected. The volumetric enzyme activity reached a maximum around D=0.3–0.4 h−1 in glucose-limited continuous cultures. However, it varied strongly (together with microorganism morphology) even after retention times ≥8 at any D tested (0.035–0.44 h−1) though the rest of the culture variables remained fairly constant. No correlation between morphology and enzyme activity could be obtained. Enzyme production was poor in urea- and vitamin-limited continuous cultures. In all cases, biomass and CO2 accounted for ≅100% of carbon recovery though Y; x/s values were different.
format Articulo
Articulo
author Cavalitto, Sebastián Fernando
Hours, Roque Alberto
Mignone, Carlos Fernando
author_facet Cavalitto, Sebastián Fernando
Hours, Roque Alberto
Mignone, Carlos Fernando
author_sort Cavalitto, Sebastián Fernando
title Growth and protopectinase production of <i>Geotrichum klebahnii</i> in batch and continuous cultures with synthetic media
title_short Growth and protopectinase production of <i>Geotrichum klebahnii</i> in batch and continuous cultures with synthetic media
title_full Growth and protopectinase production of <i>Geotrichum klebahnii</i> in batch and continuous cultures with synthetic media
title_fullStr Growth and protopectinase production of <i>Geotrichum klebahnii</i> in batch and continuous cultures with synthetic media
title_full_unstemmed Growth and protopectinase production of <i>Geotrichum klebahnii</i> in batch and continuous cultures with synthetic media
title_sort growth and protopectinase production of <i>geotrichum klebahnii</i> in batch and continuous cultures with synthetic media
publishDate 2000
url http://sedici.unlp.edu.ar/handle/10915/138739
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AT hoursroquealberto growthandprotopectinaseproductionofigeotrichumklebahniiiinbatchandcontinuouscultureswithsyntheticmedia
AT mignonecarlosfernando growthandprotopectinaseproductionofigeotrichumklebahniiiinbatchandcontinuouscultureswithsyntheticmedia
bdutipo_str Repositorios
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