Sorbed Anthracene Degradation by Sophorolipid Producing Yeasts

The organic pollutan adsorption/desorption process by microbial degradation had been less studied than metal ones. The sorption assays alone did not predict desorption, due to hysteresis, irreversibility, fixed compounds in different sites, with diverse desorption rates. Most of the studies dealt wi...

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Autores principales: Romero, María Cristina, Chiaravalli, Juan Carlos, Reinoso, Enso Hugo
Formato: Articulo
Lenguaje:Español
Publicado: 2016
Materias:
Veterinaria
Anthracene
bioavailability
biodegradation
Candida parasilopsis
desorption model Pichia anomala
Rhodothorula mucilaginosa
sophorolipid
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/138613
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-138613
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Español
topic Veterinaria
Anthracene
bioavailability
biodegradation
Candida parasilopsis
desorption model Pichia anomala
Rhodothorula mucilaginosa
sophorolipid
spellingShingle Veterinaria
Anthracene
bioavailability
biodegradation
Candida parasilopsis
desorption model Pichia anomala
Rhodothorula mucilaginosa
sophorolipid
Romero, María Cristina
Chiaravalli, Juan Carlos
Reinoso, Enso Hugo
Sorbed Anthracene Degradation by Sophorolipid Producing Yeasts
topic_facet Veterinaria
Anthracene
bioavailability
biodegradation
Candida parasilopsis
desorption model Pichia anomala
Rhodothorula mucilaginosa
sophorolipid
description The organic pollutan adsorption/desorption process by microbial degradation had been less studied than metal ones. The sorption assays alone did not predict desorption, due to hysteresis, irreversibility, fixed compounds in different sites, with diverse desorption rates. Most of the studies dealt with bacteria rather than filamentous fungi and yeasts. So, our aims were to isolate yeasts from polluted sediments, to quantify its potential to uptake anthracene (An) and to evaluate the bioavailability by a desorption model. Yeasts were isolated from hydrocarbon-polluted samples, 40-isolates grew in anthracene-plates. Molecular characterization was achieved by sequence analysis of the ITS1-5.8S rRNA-ITS4 and 26S rRNA regions; morphological and physiological determination were also done. Candida parasilopsis , Pichia anomala and Rhodothorula mucilaginosa were the prevalent yeasts. An-degradation was assessed in soil-systems with 0, 50, 100, 150, 200 and 250 I¼g An/l, 3 differentes sorbens types, organic carbon, organic nitrogen, PAHs, sand:silt:clay, pH and cation exchange capacity. Sophorolipids excretion were confirmed by HPLC, UV-detector with active fraction at 9.669 min (RT 9.646 min = sophorolipid-standard). A desorption model with equilibrium, nonequilibrium and nondesorption areas, was applied to explain the experimental data, An-transformation was greater in the organic liquid-phase than in the soil-sorbed ones; the desorption-coefficients and soil components were negatively correlated with the kinetic parameters. The An-release depended on the sophorolipid excretion, soil matrix and particles sizes. Desorption parameters significantly fitted the yeast uptake, with R 2 = 0.97, R 2 = 0.90 and R 2 = 0.97 for C. parasilopsis , P. anomala and R. mucilaginosa, respectively.
format Articulo
Articulo
author Romero, María Cristina
Chiaravalli, Juan Carlos
Reinoso, Enso Hugo
author_facet Romero, María Cristina
Chiaravalli, Juan Carlos
Reinoso, Enso Hugo
author_sort Romero, María Cristina
title Sorbed Anthracene Degradation by Sophorolipid Producing Yeasts
title_short Sorbed Anthracene Degradation by Sophorolipid Producing Yeasts
title_full Sorbed Anthracene Degradation by Sophorolipid Producing Yeasts
title_fullStr Sorbed Anthracene Degradation by Sophorolipid Producing Yeasts
title_full_unstemmed Sorbed Anthracene Degradation by Sophorolipid Producing Yeasts
title_sort sorbed anthracene degradation by sophorolipid producing yeasts
publishDate 2016
url http://sedici.unlp.edu.ar/handle/10915/138613
work_keys_str_mv AT romeromariacristina sorbedanthracenedegradationbysophorolipidproducingyeasts
AT chiaravallijuancarlos sorbedanthracenedegradationbysophorolipidproducingyeasts
AT reinosoensohugo sorbedanthracenedegradationbysophorolipidproducingyeasts
bdutipo_str Repositorios
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