Sexual dimorphism in the hypothalamo-pituitary-adrenal (HPA) axis and TNFα responses to phospholipase A2-related neurotoxin (from crotalus durissus terrificus) challenge

Neuroendocrine-immune interactions are vital for the individual’s survival in certain physiopathological conditions such as sepsis and tissular injury. It is known that several snake venoms (SV) are potent neurotoxic compounds and that their main component is a specific phospholipase type 2 (PLA2)....

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Autores principales: Chisari, Andrea Nancy, Gaillard, R. C., Giovambattista, Andrés, Voirol, Marie-Jeanne, Piermaría, Judith Araceli, Spinedi, Eduardo Julio
Formato: Articulo
Lenguaje:Inglés
Publicado: 2000
Materias:
Bioquímica
PLA2
Glucose
ACTH
Glucocorticoid
TNFα
Inflammatory stress
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/138348
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-138348
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Bioquímica
PLA2
Glucose
ACTH
Glucocorticoid
TNFα
Inflammatory stress
spellingShingle Bioquímica
PLA2
Glucose
ACTH
Glucocorticoid
TNFα
Inflammatory stress
Chisari, Andrea Nancy
Gaillard, R. C.
Giovambattista, Andrés
Voirol, Marie-Jeanne
Piermaría, Judith Araceli
Spinedi, Eduardo Julio
Sexual dimorphism in the hypothalamo-pituitary-adrenal (HPA) axis and TNFα responses to phospholipase A2-related neurotoxin (from crotalus durissus terrificus) challenge
topic_facet Bioquímica
PLA2
Glucose
ACTH
Glucocorticoid
TNFα
Inflammatory stress
description Neuroendocrine-immune interactions are vital for the individual’s survival in certain physiopathological conditions such as sepsis and tissular injury. It is known that several snake venoms (SV) are potent neurotoxic compounds and that their main component is a specific phospholipase type 2 (PLA2). It has been recently described that the venom from crotalus durissus terrificus (SV) possesses a cytotoxic effect in different in vitro and in vivo animal models. In the present study we investigated whether SV is able to stimulate both TNFα and neuroendocrine functions in a sexual dimorphic fashion. For this purpose the modulatory role of endogenous sex steroids during neurotoxemia was evaluated. Our results indicate that SV (25 μg/animal) stimulates the hypothalamo-pituitary-adrenal axis and TNFα secretion when administered (ip) to adult male mice, such responses were characterized by a time-related enhance in plasma glucose, ACTH, corticosterone and TNFα levels. SV-stimulated glycemia, corti-costeronemia and adrenal glucocorticoid were sexually dimorphic. Twenty-day gonadectomized mice showed a similar sexual dimorphism to that found in intact animals, however, they additionally showed a sexual dimorphic pattern in cytokine release in plasma 30 min post-SV. Estradiol (E2) treatment, in gonadectomized mice, abolished some characteristics of the sexual dimorphism, such as hyperglycemia, hypercorticosteronemia and hypercytokinemia. Finally, in vitro experiments indicate that: a) gonadectomy increased spontaneous and SV-stimulated cytokine output by incubated peripheral mononuclear cells (PMNC), regardless of the sex; and b) despite E2 treatment, in gonadectomized, did not modify the pattern of basal and SV-elicited TNFα secretion induced by orchidectomy, fully reversed the enhance in basal and SV-stimulated cytokine release found after ovariectomy alone. Our results further indicate that neurotoxemia, due to SV challenge, induces several symptoms common to those of inflammatory stress; they also strongly support that both gender and endogenous sex steroids are responsible for neuroendocrine-immunological sexual dimorphism.
format Articulo
Articulo
author Chisari, Andrea Nancy
Gaillard, R. C.
Giovambattista, Andrés
Voirol, Marie-Jeanne
Piermaría, Judith Araceli
Spinedi, Eduardo Julio
author_facet Chisari, Andrea Nancy
Gaillard, R. C.
Giovambattista, Andrés
Voirol, Marie-Jeanne
Piermaría, Judith Araceli
Spinedi, Eduardo Julio
author_sort Chisari, Andrea Nancy
title Sexual dimorphism in the hypothalamo-pituitary-adrenal (HPA) axis and TNFα responses to phospholipase A2-related neurotoxin (from crotalus durissus terrificus) challenge
title_short Sexual dimorphism in the hypothalamo-pituitary-adrenal (HPA) axis and TNFα responses to phospholipase A2-related neurotoxin (from crotalus durissus terrificus) challenge
title_full Sexual dimorphism in the hypothalamo-pituitary-adrenal (HPA) axis and TNFα responses to phospholipase A2-related neurotoxin (from crotalus durissus terrificus) challenge
title_fullStr Sexual dimorphism in the hypothalamo-pituitary-adrenal (HPA) axis and TNFα responses to phospholipase A2-related neurotoxin (from crotalus durissus terrificus) challenge
title_full_unstemmed Sexual dimorphism in the hypothalamo-pituitary-adrenal (HPA) axis and TNFα responses to phospholipase A2-related neurotoxin (from crotalus durissus terrificus) challenge
title_sort sexual dimorphism in the hypothalamo-pituitary-adrenal (hpa) axis and tnfα responses to phospholipase a2-related neurotoxin (from crotalus durissus terrificus) challenge
publishDate 2000
url http://sedici.unlp.edu.ar/handle/10915/138348
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