Inhibiting effect of a hepatoma extract on the mitotic rate of regenerating liver
Aqueous tumor extracts were prepared by the homogenization of a fast-growing, undifferentiated, transplantable malignant murine hepatoma in distilled water. After centrifugation, an aliquot of 0.01 ml of the supernatant g body weight was injected intraperitoneally into partially hepatectomized mice....
Guardado en:
| Autores principales: | , , |
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| Formato: | Articulo |
| Lenguaje: | Inglés |
| Publicado: |
1986
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| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/138276 |
| Aporte de: |
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I19-R120-10915-138276 |
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| record_format |
dspace |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Ciencias Médicas Chalone Colcemid Hepatoma extract Inhibiting effects Mitotic rates Regenerating liver |
| spellingShingle |
Ciencias Médicas Chalone Colcemid Hepatoma extract Inhibiting effects Mitotic rates Regenerating liver Echave Llanos, Julián Miguel Flores Badrán, Amado Moreno, Félix R. Inhibiting effect of a hepatoma extract on the mitotic rate of regenerating liver |
| topic_facet |
Ciencias Médicas Chalone Colcemid Hepatoma extract Inhibiting effects Mitotic rates Regenerating liver |
| description |
Aqueous tumor extracts were prepared by the homogenization of a fast-growing, undifferentiated, transplantable malignant murine hepatoma in distilled water. After centrifugation, an aliquot of 0.01 ml of the supernatant g body weight was injected intraperitoneally into partially hepatectomized mice. Control animals were injected with saline. Groups of mice were killed at various times in relation to the hepatectomy. Four h before killing the animals were given Colcemid (1 μg/g body weight). The number of Colcemid-arrested mitoses in the hepatocytes and in the littoral cells, respectively, were counted in 140 microscopic fields. The extract significantly inhibited the mitotic rate in hepatocytes when the injection was given between 22 h before, and up to 26 h after hepatectomy. In the littoral cells, a slight initial stimulation was followed by a slight but significant inhibition which occurred when the injection was given at hepatectomy or until 18 h after hepatectomy. The effect was not modified by exposing the extracts to temperatures of 47° C for 30 min or 22° C for 24 h, but 10 min of boiling destroyed their inhibitory effect. Lyophilization and storing at —18° C for up to 4 weeks did not modify the effect. The mitosis-inhibiting effect was also measurable when the extract was injected subcutaneously. There was an almost linear dose-response curve. The results are discussed in relation to circadian rhythms, the pattern of liver cell proliferation after hepatectomy, and recent similar reports from the literature. The conclusion is drawn that extracts of a hepatoma contain one or more growth-inhibitory factors significantly active on regenerating liver cells, and less significantly on littoral cells. |
| format |
Articulo Articulo |
| author |
Echave Llanos, Julián Miguel Flores Badrán, Amado Moreno, Félix R. |
| author_facet |
Echave Llanos, Julián Miguel Flores Badrán, Amado Moreno, Félix R. |
| author_sort |
Echave Llanos, Julián Miguel |
| title |
Inhibiting effect of a hepatoma extract on the mitotic rate of regenerating liver |
| title_short |
Inhibiting effect of a hepatoma extract on the mitotic rate of regenerating liver |
| title_full |
Inhibiting effect of a hepatoma extract on the mitotic rate of regenerating liver |
| title_fullStr |
Inhibiting effect of a hepatoma extract on the mitotic rate of regenerating liver |
| title_full_unstemmed |
Inhibiting effect of a hepatoma extract on the mitotic rate of regenerating liver |
| title_sort |
inhibiting effect of a hepatoma extract on the mitotic rate of regenerating liver |
| publishDate |
1986 |
| url |
http://sedici.unlp.edu.ar/handle/10915/138276 |
| work_keys_str_mv |
AT echavellanosjulianmiguel inhibitingeffectofahepatomaextractonthemitoticrateofregeneratingliver AT floresbadranamado inhibitingeffectofahepatomaextractonthemitoticrateofregeneratingliver AT morenofelixr inhibitingeffectofahepatomaextractonthemitoticrateofregeneratingliver |
| bdutipo_str |
Repositorios |
| _version_ |
1764820457014427649 |