Variations of the Envelope Composition of Bacillus subtilis During Growth in Hyperosmotic Medium

The envelope properties of B. subtilis cultures grown in LB and LBN hyperosmotic media (LB + 1.5 M NaCl) were compared. Since hypertonic cultures showed a Spo-phenotype, a Spo-mutant grown in LB was also analyzed. LBN cultures showed extensive filamentation and presented different sensitivities towa...

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Autores principales: López, Claudia S., Heras, Horacio, Ruzal, Sandra M., Sanchez-Rivas, Carmen, Rivas, Emilio A.
Formato: Articulo
Lenguaje:Inglés
Publicado: 1998
Materias:
Biología
Química
Bacillus subtilis
Hyperosmotic Medium
hypertonic cultures
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/137925
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-137925
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Química
Bacillus subtilis
Hyperosmotic Medium
hypertonic cultures
spellingShingle Biología
Química
Bacillus subtilis
Hyperosmotic Medium
hypertonic cultures
López, Claudia S.
Heras, Horacio
Ruzal, Sandra M.
Sanchez-Rivas, Carmen
Rivas, Emilio A.
Variations of the Envelope Composition of Bacillus subtilis During Growth in Hyperosmotic Medium
topic_facet Biología
Química
Bacillus subtilis
Hyperosmotic Medium
hypertonic cultures
description The envelope properties of B. subtilis cultures grown in LB and LBN hyperosmotic media (LB + 1.5 M NaCl) were compared. Since hypertonic cultures showed a Spo-phenotype, a Spo-mutant grown in LB was also analyzed. LBN cultures showed extensive filamentation and presented different sensitivities toward phage infection (φ29 and φ105), or antibiotics whose targets are at wall (lysozyme, penicillin G) or membrane level (polymyxin B, phosphonomycin). Results of the biochemical composition revealed that during hyperosmotic growth, the cell wall increased in thickness, and among the membrane lipids, glycolipid and cardiolipin increased in parallel with a decrease in phosphatidylglycerol. The fatty acid composition was also modified, and an increase in saturated straight chain with a decrease of saturated iso-branched fatty acids was observed. The increase of monounsaturated 18-1 (ω-9) fatty acid was probably related to the absence of sporulation observed in hypertonic media, since its increase has been shown to inhibit the KinA sensor of sporulation. The significance of the other wall and membrane composition variations (and hydrophobic surface properties) in relation to the osmotic adaptation are discussed.
format Articulo
Articulo
author López, Claudia S.
Heras, Horacio
Ruzal, Sandra M.
Sanchez-Rivas, Carmen
Rivas, Emilio A.
author_facet López, Claudia S.
Heras, Horacio
Ruzal, Sandra M.
Sanchez-Rivas, Carmen
Rivas, Emilio A.
author_sort López, Claudia S.
title Variations of the Envelope Composition of Bacillus subtilis During Growth in Hyperosmotic Medium
title_short Variations of the Envelope Composition of Bacillus subtilis During Growth in Hyperosmotic Medium
title_full Variations of the Envelope Composition of Bacillus subtilis During Growth in Hyperosmotic Medium
title_fullStr Variations of the Envelope Composition of Bacillus subtilis During Growth in Hyperosmotic Medium
title_full_unstemmed Variations of the Envelope Composition of Bacillus subtilis During Growth in Hyperosmotic Medium
title_sort variations of the envelope composition of bacillus subtilis during growth in hyperosmotic medium
publishDate 1998
url http://sedici.unlp.edu.ar/handle/10915/137925
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