Comparison of the protective effects of ischemic preconditioning and the Na+ /H+ exchanger blockade

The protective effects of ischemic preconditioning (IP) and Na+/H+ exchanger blockade (NHEb) by two blockers [ethylisopropylamiloride (EIPA) and HOE 642] were compared in the isovolumic perfused rat heart. The impairment in systolic and diastolic function detected in control ischemic hearts (C) expo...

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Detalles Bibliográficos
Autores principales: Mosca, Susana María, Cingolani, Horacio Eugenio
Formato: Articulo
Lenguaje:Inglés
Publicado: 2000
Materias:
Ciencias Exactas
Química
Ischemia
Reperfusión
Contracture
Contractility
Ischemic preconditioning
Na+/H+ exchanger
HOE 642
Protein kinase C
Chelerythrine
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/137831
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-137831
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Química
Ischemia
Reperfusión
Contracture
Contractility
Ischemic preconditioning
Na+/H+ exchanger
HOE 642
Protein kinase C
Chelerythrine
spellingShingle Ciencias Exactas
Química
Ischemia
Reperfusión
Contracture
Contractility
Ischemic preconditioning
Na+/H+ exchanger
HOE 642
Protein kinase C
Chelerythrine
Mosca, Susana María
Cingolani, Horacio Eugenio
Comparison of the protective effects of ischemic preconditioning and the Na+ /H+ exchanger blockade
topic_facet Ciencias Exactas
Química
Ischemia
Reperfusión
Contracture
Contractility
Ischemic preconditioning
Na+/H+ exchanger
HOE 642
Protein kinase C
Chelerythrine
description The protective effects of ischemic preconditioning (IP) and Na+/H+ exchanger blockade (NHEb) by two blockers [ethylisopropylamiloride (EIPA) and HOE 642] were compared in the isovolumic perfused rat heart. The impairment in systolic and diastolic function detected in control ischemic hearts (C) exposed to 20 min of ischemia and 30 min of reperfusion was diminished in similar extent by IP and by NHEb with EIPA and HOE 642. At the end of the reperfusion period +dP/dtmax values were 57±9% in C hearts and 94±6%, 82±6% and 104±6% after IP and NHEb with EIPA and HOE 642, respectively. A depletion of ATP levels detected in C hearts after reperfusion (from 20.2±0.8 µmol/g dry weight before ischemia to 6.9±0.7 µmol/g dry weight) was partially prevented by both IP and NHEb with EIPA (9.2±0.7 µmol/g dry weight and 11.1±0.5 µmol/g dry weight, respectively). The ischemic contracture (IC), assessed by the left ventricular end diastolic pressure (LVEDP), observed in C hearts (35±4 mmHg) was not decreased by IP (40±4 mmHg) but it was prevented by NHEb (18±4 mmHg and 10±3 mmHg with EIPA and HOE 642, respectively). The ATP levels at the end of the ischemic period were similar in C and IP hearts (4.1±0.2 µmol/g dry wt vs. 3.3±0.4 µmol/g dry wt) but they were significantly higher after NHEb with HOE 642 (7.0±1.0 µmol/g dry wt). PKC inhibition by chelerythrine abolished the protection induced by IP after reperfusion although not the improvement induced by NHEb with EIPA.
format Articulo
Articulo
author Mosca, Susana María
Cingolani, Horacio Eugenio
author_facet Mosca, Susana María
Cingolani, Horacio Eugenio
author_sort Mosca, Susana María
title Comparison of the protective effects of ischemic preconditioning and the Na+ /H+ exchanger blockade
title_short Comparison of the protective effects of ischemic preconditioning and the Na+ /H+ exchanger blockade
title_full Comparison of the protective effects of ischemic preconditioning and the Na+ /H+ exchanger blockade
title_fullStr Comparison of the protective effects of ischemic preconditioning and the Na+ /H+ exchanger blockade
title_full_unstemmed Comparison of the protective effects of ischemic preconditioning and the Na+ /H+ exchanger blockade
title_sort comparison of the protective effects of ischemic preconditioning and the na+ /h+ exchanger blockade
publishDate 2000
url http://sedici.unlp.edu.ar/handle/10915/137831
work_keys_str_mv AT moscasusanamaria comparisonoftheprotectiveeffectsofischemicpreconditioningandthenahexchangerblockade
AT cingolanihoracioeugenio comparisonoftheprotectiveeffectsofischemicpreconditioningandthenahexchangerblockade
bdutipo_str Repositorios
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